N-氨甲酰谷氨酸对猪胎盘滋养层细胞增殖及基因表达的影响

Effect of N-carbamylglutamate on cell proliferation and gene expression in pig placenta trophoblast cells

N-氨甲酰谷氨酸(NCG)对母猪胎盘发育和功能存在积极地调控作用。为研究NCG对母猪胎盘调控的作用机理,使用猪胎盘滋养层细胞(pTr)作为模型,用NCG体外处理猪pTr,研究NCG对细胞增殖和胎盘功能相关基因表达的影响。结果显示:1~25 mmol/L的NCG可以促进猪pTr细胞增殖1.2~1.5倍(P<0.05),10 mmol/L的NCG处理猪pTr后可显著上调CDK4、CCND1、SLC2A1和CAT基因的表达水平(P<0.05)。可见,NCG通过调控细胞周期、葡萄糖转运和抗氧化应激促进猪pTr细胞增殖。

N-carbamylglutamate(NCG)had a positive effect on placental development and function in sow.To reveal the mechanism of NCG regulation on placental function,this research used pig placental trophoblast cells(pTr)as an in vitro model to evaluate the effect of NCG on cell proliferation and related gene expression.The result showed that NCG(1-25 mmol/L)increased pTr cell proliferation 1.2 to 1.5 folds(P<0.05),in which 10 mmol/L NCG upregulated CDK4,CCND1,SLC2A,and CAT gene expressions(P<0.05).The result demonstrated that NCG could stimulate pTr cell proliferation through upregulating genes related with cell cycle,glucose transportation and antioxidant stress processes.