视网膜中央静脉阻塞与凝血酶原基因G20210A及凝血因子Ⅴ基因Leiden突变的关系

Relationships between central retinal vein occlusion and prothrombin gene G20210A and coagulation factor Ⅴ gene Leiden mutations

目的:检测视网膜中央静脉阻塞(CRVO)患者凝血酶原(PT)基因G20210A及凝血因子Ⅴ(FⅤ)基因Leiden的突变情况,并探究二者突变情况与CRVO疾病的关系。方法:选取2016年6月至2018年12月本院收治的CRVO患者82例作为研究组,选取同期健康体检者80例作为对照组,应用全自动生化分析仪检测血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)水平;应用全自动化学发光仪检测血清同型半胱氨酸(Hcy)、叶酸(FA)水平;检测PT基因G20210A和FⅤ基因Leiden的限制性长度多态性。采用向后法Logistic回归分析影响CRVO疾病发生的危险因素。结果:研究组与对照组性别、年龄、饮酒情况比较,差异无统计学意义(P> 0. 05);研究组血清Hcy、TC、TG、HDL-C、LDL-C检测值显著高于对照组(P <0. 05),FA检测值显著低于对照组(P <0. 05)。研究组与对照组均仅检测到PT基因G20210A野生型和FⅤ基因Leiden野生型,均未见突变。Logistic回归分析显示,血清FA、TC、TG、LDLC水平均可能为CRVO发生的危险因素(P <0. 05)。结论:血清FA、TC、TG、LDL-C为CRVO发生的危险因素,而PT基因G20210A和FⅤ基因Leiden突变与CRVO发生无关。

Objective: To detect the mutations of prothrombin( PT) gene G20210 A and coagulation factor Ⅴ( FⅤgene Leiden in patients with central retinal vein occlusion( CRVO),and explore the relationships between the two mutations with CRVO. Methods: 82 patients with CRVO were selected as study group from June 2016 to December2018,and 80 healthy people were selected as control group. The serum levels of total cholesterol( TC),triglyceride( TG),high density lipoprotein cholesterol( HDL-C) and low density lipoprotein cholesterol( LDL-C)were measured by automatic biochemical analyzer;the levels of serum homocysteine( Hcy) and folic acid( FA)were detected by automatic chemiluminescence analyzer;restriction fragment length polymorphisms of PT gene G20210 A and FⅤ gene Leiden were detected. Logistic regression was used to analyze the risk factors of CRVO.Results: There was no significant difference in age,gender and alcohol consumption between the study group and the control group( P > 0. 05). The detection values of Hcy,TC,TG,HDL-C and LDL-C in the study group were significantly higher than those in the control group( P < 0. 05);the detection value of FA in the study group was significantly lower than that in the control group( P < 0. 05). Only PT gene G20210 A wild type and FⅤ gene Leiden wild type were detected in both study group and control group,and no mutation was found. Logistic analysis showed that FA,TC,TG and LDL-C were all risk factors for CRVO( P < 0. 05). Conclusion: FA,TC,TG and LDL-C are risk factors for CRVO,Leiden mutation of PT gene G20210 A and FⅤ gene is not associated with CRVO.