强心安神方对阿霉素致大鼠心肌损伤模型心肌细胞能量代谢的影响

Effect of Qiangxin Anshen prescription on energy metabolism in cardiomyocytes in a rat model of myocardial injury induced by adriamycin

目的:通过研究强心安神方对阿霉素性心肌损伤大鼠模型心肌酶、心肌细胞线粒体呼吸功能和细胞色素C氧化酶活性(COX)的影响,探讨该药的作用机制。方法:将Wistar大鼠分为正常对照组、模型组、西药组及强心安神方低、高剂量组,对除正常对照组外的其余组大鼠采用腹腔注射阿霉素法制备心肌损伤模型,予药物干预4周,检测各组大鼠血清心肌酶.包括肌酸激酶同工酶(CK-MB)及乳酸脱氢酶(LDH)的表达,提取大鼠心肌细胞线粒体测定线粒体呼吸功能(呼吸控制比,RCR)及COX活性。结果:与正常对照组比较,模型组CK-MB、LDH表达均升高,COX活性增加,差异有统计学意义(P<0.01或P<0.05);强心安神方高、低剂量组大鼠各项指标与西药组比较,差异均有统计学意义(P<0.05),强心安神方高、低剂量组之间各项指标差异无统计学意义(P>0.05)。结论:强心安神方对损伤心肌细胞的保护作用可能与其降低CK-MB及LDH、改善心肌细胞线粒体呼吸功能及COX活性有关。

Objective:To investigate the mechanism of action of Qiangxin Anshen prescription by observing its effect on myocardial enzymes,mitochondrial respiratory function in cardiomyocytes,and cytochrome C oxidase(COX)activity in a rat model of myocardial injury induced by adriamycin.Methods:Wistar rats were divided into normal control group,model group,Western medicine group,and low-and high-dose Qiangxin Anshen prescription groups.All rats except those in the normal control group were given intraperitoneal injection of adriamycin to establish a model of myocardial injury.Drug intervention was performed for 4 weeks.Serum levels of myocardial enzymes,including creatine kinase-MB(CK-MB)and lactate dehydrogenase(LDH),were measured,and cardiomyocyte mitochondria was collected to measure mitochondrial respiratory function[respiratory control ratio(RCR)]and COX activity.Results:Compared with the normal control group,the model group had significant increases in the expression of CK-MB and LDH and the activity of COX(P<0.01 or P<0.05).There were significant differences in the above indices between the low-and high-dose Qiangxin Anshen prescription groups and the Western medicine group(P<0.05),while there were no significant differences in these indices between the low-and high-dose Qiangxin Anshen prescription groups(P>0.05).Conclusion:Qiangxin Anshen prescription can exert a protective effect on injured cardiomyocytes,possibly by reducing CK-MB and LDH and improving mitochondrial respiratory function and COX activity.