1株西藏H5 N1亚型禽流感病毒HA和NA基因的遗传进化分析

Genetic Evolution Analysis of HA and NA genes of a H5N1 Avian Influenza virus in Tibet

分析1株西藏H5N1亚型禽流感病毒血凝素(HA)和神经氨酸酶(NA)基因的遗传进化特征。利用RT-PCR法对H5亚型抗原阳性流感病毒核酸检测,并分子克隆、核苷酸测序,再利用DNASTAR、MEGA7.0软件进行同源性分析和基因遗传进化分析。经基因遗传进化分析表明,H5N1亚型禽流感病毒和2012年孟加拉国分离株亲缘关系最近,属于同一分支,HA蛋白裂解位点处有较多碱性氨基酸,从分子上表明西藏毒株H5N1是高致病性禽流感病毒。对西藏分离株H5N1亚型禽流感病毒HA和NA基因进行遗传进化分析,为西藏H5亚型禽流感病毒感染防控具有指导意义。

To analyze the genetic and evolutionary characteristics of Hemagglutinin(HA),Neuraminidase(NA)genes of H5N1 avian influenza virus in Tibet,the nucleic acids of H5 subtypeantigen positive influenza virus were detected by RT-PCR,cloned and sequenced,and then homology analysis and gene genetic evolution analysis were carried out by DNASTAR,MEGA7.0 software.Genetic evolution analysis showed that H5N1 subtype avian influenza virus was closely related to Bangladesh isolate in 2012 and belonged to the same branch.There were many basic amino acids at HA protein cracking sites,indicating that Tibet an strain H5N1 was a highly pathogenic avian influenza virus.The genetic evolution analysis of HA and NA genes of Tibet an isolate H5N1 avian influenza virus has guiding significance for the prevention and control of H5 avian influenza virus infection in Tibet.