C3G在结直肠癌组织中的表达变化及意义

Expression and significance of C3G in colorectal cancer tissues

目的探讨Crk SH3域结合鸟嘌呤核苷酸交换因子(C3G)在结直肠癌组织中的表达变化及意义。方法收集2012年11月~2013年12月手术切除的结直肠癌组织及其癌旁(距肿瘤边缘>5 cm)正常组织标本69例份、2019年5~8月经手术切除的31例份结直肠癌新鲜组织及其癌旁正常组织标本,分别用免疫组化法、实时荧光定量PCR技术检测以上组织中C3G蛋白及mRNA表达。对结直肠癌患者随访至2019年9月2日,分析结直肠癌组织中C3G蛋白阳性表达与患者临床病理特征及预后的关系。结果C3G蛋白定位于细胞质和细胞膜内,阳性表现为黄色或棕黄色颗粒。在结直肠癌与癌旁组织中,C3G蛋白阳性表达率分别为60.87%(42/69)、34.78%(24/69),比较差异有统计学意义(χ2=9.409,P<0.05)。结直肠癌组织中C3G mRNA相对表达量是癌旁正常组织的(5.86±2.03)倍,二者比较差异有统计学意义(P<0.05)。C3G蛋白阳性表达与结直肠癌TNM分期(χ2=7.912,P=0.005)、肿瘤浸润深度(χ2=4.242,P<0.039)、淋巴结转移(χ2=14.621,P<0.001)、CEA水平(χ2=12.503,P<0.001)有关,与患者年龄、性别无关(P均>0.05)。通过Kaplan-Meier生存曲线分析,69例结直肠癌患者中C3G蛋白阳性表达与阴性表达患者术后5年生存率分别为32.43%、60.0%,二者比较差异有统计学意义(χ2=4.612,P<0.05)。结论C3G在结直肠癌组织中呈高表达,其高表达与结直肠癌发生发展及不良预后有关。

Objective To investigate the expression and significance of Crk SH3-domain-binding guanine-nucleotide-releasing factor(C3G)in the colorectal cancer tissues.Methods Sixty-nine cases of colorectal cancer tissues and their adjacent normal tissues(5 cm away from the edge of the tumor)surgically removed from November 2012 to December 2013,and 31 cases of fresh colorectal cancer tissues and their adjacent normal tissues surgically removed from May 2019 to August 2019,were collected.Using immunohistochemistry and real-time quantitative PCR to detected the expression of C3G protein and mRNA.The patients with colorectal cancer were followed up until September 2,2019,to analyze the relationships of the positive expression of C3G protein in colorectal cancer tissues with the patients'clinicopathological characteristics and prognosis.Results C3G protein was located in cytoplasm and cell membrane,and the positive appearance was yellow or brown yellow granules.Positive expression rates of C3G protein were 60.87%(42/69)and 34.78%(24/69)respectively,in the colorectal cancer and adjacent tissues,and the difference was statistically significant(χ2=9.409,P<0.05).The relative expression of C3G mRNA in the colorectal cancer was 5.86±2.03 times higher than that in the adjacent normal tissues,and the difference was statistically significant(P<0.05).The positive expression of C3G protein was related to TNM staging(χ2=7.912,P=0.005),invasion depth(χ2=4.242,P<0.039),lymph node metastasis(χ2=14.621,P<0.001),and CEA level(χ2=12.503,all P<0.001),but was not to age or gender(both P>0.05).Kaplan-meier survival curve analysis showed that the 5-year survival rates of patients with positive and negative C3G protein expression in 69 patients with colorectal cancer were 32.43%(12/37)and 60.0%(15/25)respectively,and the differences were statistically significant(χ2=4.612,all P<0.05).Conclusions C3G is highly expressed in the colorectal cancer tissues,and the high expression is associated with the development of colorectal cancer and its poor prognosis.