Ropalladins类似物对宫颈癌Caski细胞增殖、凋亡的影响

Effects of Ropalladins analog on proliferation and apoptosis of cervical cancer Caski cells

目的探讨海洋生物碱Ropalladins类似物(化学名:E-N-叔丁基-2-对氯苯甲酰基-1-异丙基-4-对氯苄叉基-5吡咯烷酮-2酰胺,以下简称为化合物a)对宫颈癌Caski细胞增殖、凋亡的影响及其可能机制。方法选取宫颈癌Caski细胞系,设置B组和E、F、G组,分别给予DMSO及终浓度为12.5、25、50μmol/L的化合物a,培养48 h后收集各组细胞,MTT法测定细胞生长抑制率,Annexin V-FITC/PI双染色法测定细胞凋亡率,qRT-PCR法检测细胞E6、E7 mRNA的表达。结果与B组相比,E、F、G组生长抑制率均增高,细胞凋亡率均增高,E6、E7 mRNA表达均降低(P均<0.05)。结论化合物a能有效地抑制Caski细胞增殖并促进其凋亡,其机制可能与抑制Caski细胞中E6、E7 mRNA的表达有关。

Objective To investigate the effects of the analog of Ropalladins(E-N-tert-butyl-2-p-chlorobenzoyl-1-isopropyl-4-p-chlorobenzylidene-5pyrrolidinone-2-amide,hereinafter to be referred as compound a)on the proliferation and apoptosis of human cervical Caski cells and the underlying mechanisms.Methods The Caski cells were selected and randomly divided into four groups(groups B,E,F and G),which were added with DMSO,12.5,25,and 50μmol/L compound a.The Caski cells were collected after being cultured for 48 h.MTT assay was used to observe the growth inhibition rate of Caski cells.Annexin V-FITC/PI assay was used to evaluate the apoptosis rate of Caski cells.Quantitative RT-PCR(qRT-PCR)was utilized to measure the relative expression of E6 and E7 mRNA in Caski cells.Results Compared with group B,the growth inhibition rates and the apoptosis rates of groups E,F,and G increased,and the expression of E6 and E7 mRNA decreased(all P<0.05).Conclusion Compound a can inhibit the proliferation and induce the apoptosis of Caski cells significantly,and the possible mechanism may be associated with the decreased expression of E6 and E7 mRNA.