TET1过表达对宫颈癌细胞增殖迁移能力的影响

Effect of TET1 overexpression on the proliferation of cervical cancer cells

目的通过对HeLa细胞过表达TET1基因,探究TET1基因对宫颈癌细胞增殖迁移能力的影响。方法采用TALE-VP64系统构建TET1过表达质粒,转染宫颈癌HeLa细胞,四唑盐(MTT)比色法监测细胞增殖状况,划痕试验检测细胞迁移能力的变化,Transwell试验检测HeLa细胞侵袭能力的变化。结果 MTT法检测HeLa细胞增殖状况,TET1过表达的Clone 1和Clone 2细胞增殖能力明显弱于野生型HeLa细胞(P<0.05)。Transwell试验检测TET1过表达Clone 1及Clone 2 HeLa细胞穿过magtrigel胶及小室的数量分别是(21±5)和(22±6)个/视野,而野生型HeLa细胞组为(38±7)个/视野,与野生型HeLa细胞组比较,差异有统计学意义(P<0.05)。划痕试验检测TET1过表达组(Clone 1、Clone 2)细胞和野生型HeLa组细胞24 h及48 h的迁移率,后者明显高于前者(P<0.01)。TET1过表达能够抑制HeLa细胞的增殖、侵袭、迁移等能力。结论 TET1基因过表达对宫颈癌HeLa细胞有抑癌基因的作用。

Objective To investigate the effect of TET1 gene on the proliferation and migration of HeLa cells. Methods The overexpression plasmid of TET1 was constructed by transcription activator-like effectors(TALE)-vp64 system and transfected into cervical cancer HeLa cells. The proliferation of HeLa cells was monitored by thiazolyl blue tetrazolium bromide(MTT), the migration ability of HeLa cells was detected by scratch test, and the invasion ability of HeLa cells was detected by Transwell.Results MTT assay showed that the proliferation ability of TET1 overexpressed cells was significantly lower than that of wild-types(P<0.05).The numbers of HeLa cells passed through the matrigel were(Clone 1, 21±5) and(Clone 2,22±6) in TET1 overexpresed cells, and(38±7) in wild-type HeLa group,the difference between the two group was statistically significant(P<0.05). The migration rates of HeLa cell in TET1 overexpressed(Clone 1, Clone 2) and the wild-type group at 24 h and 48 h were detected by scratch test, the latter was significantly higher than the former(P<0.01).TET1 overexpression could restrain the ability of proliferation, invasion and migration of HeLa cells. Conclusion Overexpression of TET1 gene can inhibit the biological behavior of cervical cancer cells.