东云‘Corderoyi’的EMS离体诱变及突变体筛选

EMS mutagenesis in vitro and mutant screening of Echeveria agavoides ’Corderoyi’

对多肉植物东云系‘Corderoyi’进行不同浓度和浸泡时间的甲基磺酸乙酯(EMS)诱变处理,研究最适合的处理方法、获得不同类型突变体.实验中以‘Corderoyi’的花梗为外植体进行不同浓度EMS浸泡处理,再诱导分化培养,观察表型变异,进行突变体筛选.结果表明‘Corderoyi’外植体诱变的最佳处理是0.05%EMS溶液浸泡6 h,外植体致死率为64.86%,突变率可以达到14.50%.研究筛选得到了4类表型变异稳定、具有观赏价值的多肉植物突变体,可以丰富其种质资源,为景天科多肉植物的诱变提供参考.

In order to determine the optimum treatment method and obtain different types of mutants,Echeveria agavoides’Corderoyi’were treated by ethyl methyl sulfonate(EMS)under different concentrations and soaking time in this study.The pedicels of’Corderoyi’were treated by EMS immersion as explants,and differentiation was induced by tissue culture.Phenotypic difference was observed to screen the mutants.The results showed that the optimum treatment for’Corderoyi’explant mutagenesis was at 0.05%EMS immersion for6 hours.The fatality rate and mutation rate of explants were 64.86%and 14.50%,respectively.Four types of mutants with stable phenotypic variation and ornamental value were screened.It could enrich their germplasm resources and serve as reference for the mutagenesis of other Crassulaceae plants.