口腔鳞癌中NF-κBp65、IL-6表达与巨噬细胞极化的关系

The relationship among NF-κB p65,IL-6 and macrophage polarization in oral squamous cell carcinoma

目的:探讨口腔鳞状细胞癌(OSCC)中核因子-κBp65(NF-κBp65)、白介素-6(IL-6)的表达及其二者与巨噬细胞极化之间的关系。方法:采用免疫组织化学SP法检测在15例正常组织及42例OSCC组织中NF-κBp65、IL-6的表达及CD68^+、CD163^+巨噬细胞的浸润情况,并结合临床病理参数进行对比分析。结果:在OSCC中,NF-κBp65、IL-6的表达及CD68^+、CD163^+巨噬细胞的浸润数量均高于正常组织(P<0.05);NF-κBp65的表达、CD68^+及CD163^+巨噬细胞的浸润数量与淋巴结转移有关(P<0.05);NF-κBp65与IL-6、CD68^+、CD163^+巨噬细胞在OSCC中的表达或浸润数量均呈正相关(P<0.05);CD68^+、CD163^+巨噬细胞的浸润数量呈正相关(P<0.05)。结论:在OSCC中,NF-κBp65和IL-6的表达增高并参与了巨噬细胞极化过程,三者与肿瘤的侵袭与转移密切相关,可能在OSCC发展中存在相互促进作用。

Objective:To investigate the relationship among NF-κB p65,IL-6 and macrophage polarization in oral squamous cell carcinoma(OSCC).Methods:The immunohistochemical technique with SP method was used to detect the expression of NF-κBp65,IL-6,CD68+macrophages and CD163+macrophages in 42 cases of OSCC tissues and 15 normal oral mucosa tissues.The correlation of the indicators with the clinicopathological data was analyzed.Results:In the OSCC group,the expression of NF-κBp65,IL-6,CD68+macrophages and CD163+macrophages was significantly higher than that of the normal tissue group(P<0.05).The expression of NF-κBp65,CD68+and CD163+macrophages was correlated with lymph node metastasis(P<0.05).In OSCC there was a positive correlation between the expression of NF-κBp65 and IL-6(P<0.05),between the expression of NF-κBp65 and CD68+macrophages(P<0.05),between the expression of NF-κBp65 and CD163+macrophages(P<0.05),between expression of CD68+macrophages and CD163+macrophages in OSCC(P<0.05).Conclusion:In OSCC,the expression of NF-κBp65 and IL-6 is increased,the polarization of macrophages is promoted,the 3 indicators are closely related to tumor invasion and metastasis,and may promote each other in the development of OSCC.