酸性磷酸酶确证法快速检测生活饮用水中产气荚膜梭菌

Rapid Detection of Clostridium perfringens in Drinking Water by Acid Phosphatase Identification Method

自《生活饮用水卫生标准》(GB 5749-2006)附录A中对微生物指标产气荚膜梭菌提出参考限值要求后,至今未出台相关检测标准。针对此问题,参考现行有效的食品类产气荚膜梭菌检测标准,在经传统生化培养分离的前提下,研究对比了两种鉴定产气荚膜梭菌的方法--标准表型鉴定法和酸性磷酸酶确证法。结果表明,酸性磷酸酶确证法可用于鉴定饮用水中产气荚膜梭菌。引入酸性磷酸酶确证法可简化饮用水中产气荚膜梭菌的检测步骤,该方法可将产气荚膜梭菌的整体检测周期缩短至48 h,一定程度上提高了检测饮用水中产气荚膜梭菌的效率,为快速判断饮用水生物安全性打下基础。

Since the requirements for the reference limit of the microbial indicator Clostridium perfringens were put forward in appendix A of Standards for Drinking Water Quality(GB 5749-2006), no relevant testing standards have been issued. In view of this problem, referring to the existing detection standards of Clostridium perfringens in food, two methods for identification of Clostridium perfringens, e.g.standard phenotypic identification and acid phosphatase identification were studied and compared on the premise of traditional biochemical culture and isolation. The results showed that acid phosphatase identification could be used to identify Clostridium perfringens in drinking water. Introduction of acid phosphatase identification could simplify the detection steps of Clostridium perfringens in drinking water. This method can shorten the overall detection cycle of Clostridium perfringens to 48 h. So the detection efficiency of Clostridium perfringens in drinking water was improved. And furthermore, a foundation was established for rapid judgment of the drinking water biosafety.