血根碱通过上调TSP-1抑制人脐静脉内皮细胞增殖

Sanguinarine Inhibits Proliferation of Human Umbilical Vein Endothelial Cells through Up-regulating TSP-1

[目的]探讨血根碱对人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVEC)的抑制作用及机理。[方法]培养HUVEC细胞,分为对照组、血根碱组,对照组加0.9%氯化钠液,血根碱组分别加1.00μmol/L、2.00μmol/L、3.00μmol/L、4.00μmol/L血根碱,48h后应用MTT法、细胞小管形成实验、Western blot法分别检测血根碱对HUVEC细胞增殖、小管形成、以及Bax、Caspase-3和血小板反应素-1 (thrombspondin-1,TSP-1)表达的影响。[结果]与对照组相比,血根碱组各浓度均可显著抑制HUVEC细胞增殖、小管形成,促进细胞凋亡及Caspase-3、Bax和TSP-1表达增高(P<0.05)。[结论]血根碱通过上调TSP-1的表达,促进HUVEC细胞凋亡,抑制HUVEC细胞的增殖及新生血管形成,发挥抗血管生成的作用,阻碍肿瘤的发展。

[Objective] To investigate the effect and mechanism of sanguinarine on proliferation of human umbilical vein endothelial cells(HUVECs). [Methods] HUVECs were treated with1.0,2.0,3.0 and 4.0 μmol/L of sanguinarine for 48 h,and the control group was treated with normal saline. The proliferation and tubule formation of HUVECs were detected by MTT and cell tubule formation test. The expressions of Bax,Caspase-3 and thrombspondin-1(TSP-1) were detected by Western blot. [Results] Compared to the control group,the proliferation and tubule formation of HUVECs were significantly inhibited in sanguinarine-treatment groups;and the expressions of caspase-3,Bax and TSP-1 were increased(P<0.05). [Conclusion] sanguinarine can inhibit the proliferation of HUVECs and angiogenesis,which is associated with up-regulating the expression of tsp-1 and promoting the apoptosis of HUVECs.