摘要
香叶草基合成酶(Geranylgeranyl bisphosphate synthase,GGPPS)与囊泡运输过程中小G蛋白的翻译后修饰密切相关。文章选择同时具有2个Loxp位点基因和GGPPS基因的Ggpps-floxed小鼠与带有脂肪组织特异性启动子aP2的Cre小鼠aP2-Cre进行杂交,以获得脂肪组织GGPPS特异性缺失的小鼠Ad Ggpps KO。经过基因鉴定和多组织的定量聚合酶链反应(polymerase chain reaction,PCR)以及Western blot确认,得到的小鼠模型的脂肪组织GGPPS缺失。
Geranylgeranyl bisphosphate synthase(GGPPS) was closely related with vesicle trafficking and post-translational modifications of small G proteins. Ggpps-floxed mice with two Loxp genes and GGPPS gene and aP2-Cre mice with adipose tissue specific promoter aP2 were selected to generate adipose tissue GGPPS-specific knockdown mice Ad Ggpps KO. The results of genotyping, quantitative polymerase chain reaction(PCR) and Western blot showed that the expression of GGPPS in adipose tissue was reduced.
作者
薛晓萌
刘健
XUE Xiaomeng;LIU Jian(School of Food and Biological Engineering,Hefei University of Technology,Hefei 230601,China)
出处
《合肥工业大学学报（自然科学版）》
CAS
北大核心
2020年第2期270-273,共4页
Journal of Hefei University of Technology：Natural Science
基金
国家自然科学基金资助项目(31671485)
