彩色多普勒超声显示早期创伤后异位骨化的实验研究

Color Doppler ultrasound visualizes early post-traumatic heterotopic ossification in animal model

目的在动物模型中验证彩色多普勒超声检测脑创伤-烧伤-跟腱切开术后早期异位骨化。方法44只雄性SD大鼠随机分为实验组和对照组,每组22只。实验组为脑创伤-烧伤-跟腱切开异位骨化模型组,对照组仅切开跟腱处皮肤不予其他干预。建模后第2、3、4、6、8、10周分别行彩色多普勒超声和X线平片检查,并行组织学检查确定骨的形成,以第10周X线和HE染色最终确定异位骨化。结果第2周实验组跟腱超声表现为结构紊乱,内部回声不均匀,中间可见低回声区。第3周实验组跟腱处可见细小点状强回声。第4周点状强回声增多。以上各时期X线检查两组跟腱变化未发现异常。于第6周后实验组跟腱处超声可见强回声相互融合成环状。X线检查实验组跟腱处见异位骨化,对照组跟腱处无异位骨化。第10周X线及HE染色最终确定实验组跟腱处异位骨化形成。结论彩色多普勒超声在受伤后可以早期发现异位骨化,并可用来连续观察异位骨组织的成熟过程。

Objective To validate the role of color Doppler ultrasound in an animal model to detect early heterotopic ossification(HO)after brain-traumatic/burn/tenotomy.Methods Forty-four rats were randomly divided into two groups.Rats in experimental group(n=22)were operated to build brain-traumatic/burn/tenotomy model and others in control group(n=22)underwent only skin incision injury.Color Doppler ultrasound,X-ray film examination at 2,3,4,6,8 and 10 weeks post-injury were performed to follow up the progression of HO in both groups respectively.Histology was used to confirm bone formation.Results In the experimental group,disorder structure with a hypoechoiccore in treated Achilles tendon was visualized using color Doppler ultrasound in the 2nd week.Additional tiny hyperechoic foci were observed in the 3rd week,which increased in the fourth week and fused into a mineralized island in the sixth week.No obvious abnormality was found in control group at the aforementioned time point.X-ray could detect heterotopic bone tissue in the sixth week in the experimental group but not in the control group.X-ray and HE stainning had confirmed bone formation in the tenth week in the experimental group.Conclusions Color Doppler ultrasound can detect early HO and continuously follow up the progression of HO.