酶工程综合性实验项目的设计与实践

Design and Practice of Comprehensive Experiment Project of Enzyme Engineering

酶工程实验作为生物技术的主要课程之一,具有很强的实用性和应用性。本课程的目的是锻炼学生运用酶知识和技术解决问题。为此,设计了一个综合性实验项目——木瓜蛋白酶酶反应器的设计及应用,将酶的制备、酶学性质分析、酶的修饰固定、酶反应器的设计及应用等知识点进行整合,选取南方特征水果木瓜为实验对象,包括木瓜蛋白酶的制备、木瓜蛋白酶活力的测定、激活剂对木瓜蛋白酶活力的影响、壳聚糖凝胶颗粒固定化木瓜蛋白酶、酶反应器设计及酪蛋白水解物的制备等6个部分。这项实验可以培养学生综合运用酶学知识和实验技能解决问题的能力。

As one of the main courses of Biotechnology,experiment of enzyme engineering has strong practicality and application.The aim of this course is to train students to solve problems with enzyme knowledge and technique.This paper introduced a comprehensive experiment:the design and application of papain enzyme reactor.This project integrated the processes of enzyme preparation,enzyme properties analysis,enzyme modification and immobilization,and the design and application of enzyme reactor,etc.This experiment selectd papaya,a southern characteristic fruit,as the research object.It contained six sections:the preparation of papain,the determination of papain activity,the effect of activator on papain activity,the immobilized papain with chitosan gel particles,the design of enzyme reactor and the preparation of casein hydrolysates.Students′way of thinking and experiment skills in enzyme research can be fostered by this comprehensive experiment.