南粳5055及其亲本的光合特性

Photosynthetic characteristics of japonica rice cultivar Nanjing 5055 and its parents

为探究优良食味粳稻的光合特性,进一步挖掘南粳系列水稻光合潜力,对优良食味粳稻南粳5055及其母本武粳13和父本日本优质粳稻关东194的叶绿素荧光参数、类囊体膜蛋白组分与含量进行分析。结果显示:南粳5055在生殖生长期的叶绿素和类胡萝卜素含量、净光合速率、量子产额、光合性能指数高于关东194,略低于武粳13;南粳5055在完熟期的结实率与千粒质量接近武粳13,高于关东194;温和蓝绿胶电泳结果表明,南粳5055在孕穗期和灌浆期光系统I复合物含量较高;蛋白质免疫印迹显示南粳5055具有较高的Lhca1和Lhca2蛋白含量,而光系统II中心复合物和捕光色素II复合物等其他复合物含量均低于武粳13。说明南粳5055的光合特性在一定程度上与母本武粳13较为接近,南粳5055在孕穗期和乳熟期保持相对较高的光系统I复合物含量,且在生育后期具有高效的热耗散能力可以避免光抑制的发生,推测这可能是该品种能保持高效光合特性的物质和代谢基础。

In order to explore the photosynthetic characteristics and photosynthetic potentiality of Nanjing rice,the chlorophyll fluorescence parameters and the components and contents of thylakoid membrane protein of Nanjing 5055,its female parent Wujing 13 and male parent Guandong 194 were studied.The results showed that the chlorophyll content,carotenoid content,net photosynthesis rate,quantum yield and photosynthetic performance index of Nanjing 5055 were higher than those of Guandong 194 and slightly lower than those of Wujing 13 in the reproductive period.The seed setting rate and 1000-grain weight of Nanjing 5055 were higher than those of Guandong 194 and chose to those of Wujing 13 in the full stage.Blue native polyacrylamide gel electrophoresis(BN-PAGE)results showed that the contents of photosystem I complexes in Nanjing 5055 were higher at the booting and milking stages.The results of western blotting indicated that the contents of Lhca1 and Lhca2 protein in Nanjing 5055 were higher,while the contents of other complexes such as photosystem II core and light-harvesting pigment II were slightly lower than those in Wujing 13.The above results indicated that the photosynthetic performance of Nanjing 5055 was close to its female parent Wujing 13,and the contents of photosystem I complexes were higher in the booting and milk stages.Moreover,Nanjing 5055 had high heat dissipation performance to avoid light inhibition in the later growth stage,so it was speculated that those were material and metabolic basis for Nanjing 5055 to maintain high photosynthetic efficiency.