聚吡咯改性聚偏氟乙烯膜的电学特性及生物相容性

Electrical Property and Biocompatibility of Polyvinylidene Fluoride Films Modified by Polypyrrole

聚偏氟乙烯因具有生物相容性、压电性及良好的机械性能而在生物医用中备受关注。利用电化学法在聚偏氟乙烯膜表面沉积聚吡咯涂层以表面改性聚偏氟乙烯膜,研究了聚吡咯纳米颗粒和纳米线结构涂层对聚偏氟乙烯膜的表面亲水性、电学特性及生物相容性的影响。采用扫描电镜和原子力显微镜观察了纳米结构聚吡咯涂层改性聚偏氟乙烯膜的表面形貌;利用表面接触角测量仪、原子力显微镜研究了改性聚偏氟乙烯膜的亲水性和电学特性;通过体外矿化、细胞死活染色和CCK-8检测方法探讨了改性聚偏氟乙烯膜的生物相容性。结果表明:纳米结构聚吡咯涂层改性聚偏氟乙烯膜的表面亲水性、表面电势分布及生物相容性明显提高了,有利于表面类骨矿物的沉积及细胞的粘附与增殖。此外,纳米线结构聚吡咯改性的聚偏氟乙烯膜具有更高的亲水性、表面电势分布,更有利于细胞粘附与增殖。

Polyvinylidene fluoride(PVDF) has attracted much attention due to its biocompatibility, piezoelectric and favourable mechanical properties. The modification of polypyrrole coating on the surface of the PVDF film by electrochemical method was studied. The surface morphology of the nanostructured polypyrrole coating modified PVDF film was observed by scanning electron microscopy(SEM) and atomic force microscope(AFM). The surface contact angle analyzer and AFM were used to study the hydrophilicity and electrical properties of the PVDF membrane modified by the nano structure polypyrrole coating. The biocompatibility of the nanostructured polypyrrole coating modified PVDF membrane was investigated by in vitro mineralization, cell viability staining and CCK-8 assay, respectively. The results show that the surface hydrophilicity,surface potential distribution and biocompatibility of PVDF films are significantly improved by the modification of the nanostructured polypyrrole coating, and more conducive to the surface bone mineral deposition and cell adhesion and proliferation. In addition, the PVDF membrane modified by polypyrrole nanowire has higher hydrophilicity and surface potential distribution, which is more conducive to cell adhesion and proliferation.