辽诱系列玉米单倍体诱导系的选育

Breeding of LAAS-set Haploid Inducers with High Frequency Parthenogenesis in Maize

采用多个测验种逐代跟踪测定诱导率的方法,利用俄罗斯引进的单倍体诱导系资源,经过5年7代选育出辽诱系列玉米单倍体诱导系辽诱1号、辽诱2号、辽诱3号和辽诱4号。对22个品种的诱导效果试验表明,辽诱1号、辽诱2号、辽诱3号和辽诱4号的综合诱导率分别为6.85%、7.53%、6.66%和6.03%,比对照高诱五号分别高出0.6倍、0.8倍、0.6倍和0.4倍。诱导基因检测结果表明,辽诱系列诱导系具有诱导率高、花粉量大、抗病、抗倒、易繁殖等优良特性,且存在和高诱五号共同的诱导基因GRMZM2G471240,是开展玉米单倍体育种的理想诱导系。

A batch of haploid inducer resources was introduced from Russia in 2013 and used for breeding.LAAS-1,LAAS-2,LAAS-3 and LAAS-4 were bred by using multi-test materials with high frequency inducing rate(IR)through 7 generations between 5 years.The results of induction effect test for 22 entries showed that the IR of LAAS-1,LAAS-2,LAAS-3 and LAAS-4 were 6.85%(0.6 times higher than CK),7.53%(0.8 times higher than CK),6.66%(0.6 times higher than CK)and 6.03%(0.4 times higher than CK),respectively.The results of induced gene test indicated that the LAAS-set haploid inducers and CAU-5 shared the same induced gene GRMZM2 G471240.LAAS-set haploid inducers are ideal inducers for haploid breeding of maize at present in good characteristics such as high inducing rate,abundant pollen quantity,resistance to blight and lodging,easy reproduction,etc.