玉米光周期敏感性基因ZmDPS10-2启动子结合蛋白筛选研究

Screening of Photoperiod Sensitivity Gene ZmDPS10-2 Promoter’s Binding Proteins in Maize

以玉米光周期敏感基因ZmDPS10-2的核心启动子(1148 bp)为诱饵,采用酵母单杂交技术,从热带玉米自交系酵母单杂交文库中,筛选到33个与ZmDPS10-2的启动子相互作用的蛋白,并根据基因功能注释,从中选出了4个与光周期响应和逆境响应相关的蛋白PAO4、RPL23A、bZIP60和BAG。通过构建pGADT7载体做进一步回转验证,初步证实他们与候选基因启动子之间存在互作关系。结合启动子功能元件的预测,推测ZmDPS10-2候选基因可能在植物开花、ER胁迫、热胁迫等逆境调控途径中有一定的功能作用。

Using the core promoter(1148 bp) of the maize photoperiod-sensitive gene ZmDPS10-2 as a bait,and using the yeast one-hybrid technology, from the tropical maize inbred line yeast one-hybrid library, we screened 33 genes interacting with ZmDPS10-2’s promoter. According to gene annotation, we selected four genes related to photoperiod and stimulus response, including PAO4, RPL23 A, bZIP60 and BAG. These four genes were constructed into pGADT7 to confirm their interaction. It was initially confirmed that there was an interaction between these four genes and ZmDPS10-2’s promoter. Combined with the prediction of promoter functional elements,it is speculated that ZmDPS10-2 may play a certain functional role in plant flowering and stress regulation pathways such as ER stress and heat stress.