亚砷酸盐胁迫下水稻microRNA的应答功能及调控通路分析

Analysis of Function and Regulatory Pathway of Responsive microRNAs in Rice under Arsenite Stress

为明确砷胁迫下microRNA的应答功能及调控通路,本研究利用miRNA芯片检测水稻幼苗在亚砷酸盐胁迫后miRNA的表达差异。结果发现11个miRNA表达显著下调,7个显著上调,并采用RT-PCR验证了9个miRNA的差异表达。差异miRNA靶基因多编码转录因子以及重金属响应网络中的蛋白。基因本体(GO)和KEGG通路富集表明,砷胁迫下miRNA主要介导调控激素信号转导、硫代谢、氨基酸代谢和抗氧化防御。利用PLACE数据库发现miRNA上游启动子区密集分布多种金属应答元件。本研究有助于阐明水稻中砷转运和积累的分子机制,为水稻抗逆育种提供理论参考。

In order to clarify the response function and regulatory pathway of microRNA under arsenic stress,we used miRNA microarray to detect the difference of miRNA expression in rice seedlings after arsenite stress.This study found that 11 miRNAs were significantly down-regulated,7 were significantly up-regulated,and differential expressions of 9 miRNAs were verified by RT-PCR.Differential miRNA target genes encode multiple transcription factors as well as proteins in heavy metal response networks.Gene ontology(GO)and KEGG pathway enrichment indicated that miRNAs mainly mediates regulation of hormone signaling,sulfur metabolism,amino acid metabolism and antioxidant defense under arsenic stress.A large number of metal response elements were densely distributed in the upstream promoter region of the miRNA using the PLACE database.This study helps to elucidate the molecular mechanism of arsenic transport and accumulation in rice,and provides a theoretical reference for rice resistance breeding.