超高效液相色谱-串联质谱法测定杨梅中噻嗪酮残留量

Determination of Buprofezin Residues in Waxberry by UPLC-MS/MS

[目的]建立超高效液相色谱-串联质谱法测定杨梅中噻嗪酮残留量的方法。[方法]杨梅样品经乙腈提取,弗罗里硅土柱净化,超高效液相色谱-串联质谱检测。[结果]噻嗪酮在0.5~500μg/L范围内线性关系良好,相关系数为0.9952,在0.01~2.0 mg/kg添加水平下,噻嗪酮在杨梅中的平均回收率为88%~92%,相对标准偏差为2.43%~3.88%,噻嗪酮在杨梅中的定量下限为0.01 mg/kg。[结论]该方法简便、快速、准确、灵敏度高,可用于杨梅中噻嗪酮的残留检测。

[Aims]The analytical method for the determination of buprofezin residues in waxberry was developed based on ultrahigh performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS).[Methods]The samples were extracted with acetonitrile,purified with an florisil column,and determined by UPLC-MS/MS.[Results]The calibration curves for buprofezin were linear in the range of 0.5-500μg/L with correlation coefficients of 0.9952.When the spiked level ranged from 0.01 to 2 mg/kg,the average recoveries of buprofezin in waxberry were 88-92%,and the relative standard deviation(RSD)ranged from 2.43 to 3.88%.The limit of quantitation(LOQ)was 0.01 mg/kg.[Conclusions]The method is simple,rapid,accurate,and sensitive,and can be adapted for the determination of buprofezin residues in axberry.