谷子AMT基因家族的鉴定及生物信息学分析

Identification and Bioinformatics Analysis of Millet AMT Gene Family

铵态氮转运蛋白(AMT)是一种高亲和性的转运蛋白,对植物的生长和发育有着重要的调节作用。谷子作为耐贫瘠作物,鉴定谷子AMT基因家族成员及对其进行生物信息学分析,将为研究谷子AMT基因家族的分子功能奠定一定的基础。根据已经报道的拟南芥和水稻AMT基因,运用生物信息学方法在谷子xiaomi基因组上鉴定了谷子AMT基因家族成员,并分析其在染色体上的分布、理化性质和基因结构,同时基于不同组织中的基因表达量,构建谷子AMT基因的表达模式。结果表明,在谷子xiaomi基因组中共鉴定到9个AMT基因家族成员,分为4个亚族,均由α-螺旋、β-折叠、扩展链以及无规则卷曲4种形式组成;不同SiAMT基因家族间的等电点、亲水性、相对分子质量等理化性质存在差异。motif分析结果表明,Si AMT蛋白均含有motif 8、motif 6、motif 5;此外,Si1g19720.1、Si1g24430.1、Si5g39610.1、Si7g17570.1基因的启动子区检测到与植物生长发育、光照、激素、胁迫响应有关的顺式作用元件。表达谱分析结果发现,Si7g17570.1、Si3g20620.1、Si9g02830.1基因具有明显的组织表达特异性。研究结果可为深入探讨该基因家族的功能机制与表达调控提供一定的参考信息。

Ammonium nitrogen transporter(AMT)is a high-affinity transporter that plays an important role in regulating plant growth and development.As a barren-tolerant crop,identifying and biological analyzing the members of millet AMT gene family will lay a certain foundation for studying the molecular function of millet AMT gene family.Based on the reported Arabidopsis thaliana and rice AMT genes,the bioinformatics method was used to identify the members of the AMT gene family in the genome of xiaomi,and to analyze its distribution,physicochemical properties and gene structure on chromosome.In addition,the expression pattern of the millet AMT gene was constructed based on the amount of gene expression in different tissues.The result showed that a total of 9 identified AMT gene family members in the xiaomi genome were divided into four subfamilies,which were composed ofα-helix,β-sheet,extended chain and random coil.There were differences in physicochemical properties such as isoelectric point,hydrophilicity and relative molecular mass of different SiAMT genes family.motif analysis results showed that SiAMT protein contained motif 8,motif 6 and motif 5.In addition,the promoter regions of the Si1g19720.1,Si1g24430.1,Si5g39610.1 and Si7g17570.1 genes were detected cis-acting elements involved in plant growth and development,light,hormones and stress responses.The results of expression profiling revealed that the Si7g17570.1,Si3g20620.1 and Si9g02830.1 genes had significant tissue expression specificity.The results of this study will provide some reference information for further study of the functional mechanism and expression regulation of this gene family.