高效液相色谱法测定阳和汤中毛蕊花糖苷和甘草苷两种成分

Simultaneous Determination of Acteoside and Liquiritoside Contained in Yanghe Tang by HPLC

目的:采用高效液相色谱法(HPLC)同时测定阳和汤中的毛蕊花糖苷和甘草苷2种有效成分。方法:使用Nobel Kromasil C18(4.6 mm×150 mm×5μm)作为色谱柱,以乙腈和0.1%的磷酸水溶液作为流动相,梯度洗脱,流动相的流速为1 m L/min,检测波长分别为334 nm和237 nm。结果:阳和汤中毛蕊花糖苷和甘草苷的峰面积与其进样量有较好的线性关系,并且它们的相关系数均超过0.9992,所使用仪器精密度的标准差不超过2%,平均回收率均大于96%。结论:采用高效液相色谱法测定阳和汤中毛蕊花糖苷和甘草苷这两种有效成分,快速、简便、回收率高,是测定阳和汤中这两种标志性有效成分的有效方法。

Objective:To determine two kinds of active ingredients acteoside and liquiritoside simultaneously contained in Yanghe Tang by HPLC.Methods:Nobel Kromasil C18(4.6 mm×150 mm×5μm)was used as the chromatographic column,acetonitrile and 0.1%phosphoric acid aqueous solution as the mobile phase,gradient elution,velocity of flow was 1 m L/min,detection wavelengths were 334 nm and 237 nm respectively.Results:Peak areas of acteoside and liquiritoside contained in Yanghe Tang and their sample sizes showed better linear relationship,and their correlation coefficients were greater than 0.9992,precision standard deviation of the instruments was no more than 2%,average recovery rates were greater than 96%.Conclusion:The determination of two kinds of active ingredients acteoside and liquiritoside contained in Yanghe Tang by HPLC,rapid,convenient and high recovery,are the effective methods of detecting two kinds of active ingredients contained in Yanghe Tang.