miR-497促进肝癌MHCC97L细胞增殖和迁移

The miR-497 promotes proliferation and migration in MHCC97L cells

目的:探讨miR-497促进肝癌MHCC97L细胞增殖和迁移。方法:选取肝癌细胞系MHCC97L细胞转染miR-497和miR-zip后,CCK-8分析各组细胞增殖;Transwell实验分析各组细胞的迁移能力;Western blot和实时定量PCR分析各组RIPK1和NF-κB表达情况。结果:过表达miR-497细胞增殖活性和迁移能力明显提高;过表达miR-497细胞中的RIPK1mRNA和NF-κB mRNA表达增高;western blot分析得出过表达miR-497的MHCC97L细胞的RIPK1蛋白和NF-κB表达均上调。结论:miR-497通过调控RIPK1,从而激活NF-κB促进肝癌MHCC97L细胞增殖和迁移。

Objective:The miR-497 promotes proliferation and migration in MHCC97L cells.Methods:After 24 h transfection with miR-Zip or miR-497,in MHCC97L cells,the proliferation of MHCC97L was analyzed by CCK-8.The migration was detected by transwell in MHCC97L transfected with miR-Zip or miR-497.The expression of RIPK1 and NF-κB were analyzed by qPCR and western blot in MHCC97L with miR-Zip or miR-497.Results:The proliferation of MHCC97L expressed miR-497 was significantly increased than that of expressed miR-Zip MHCC97L.The migration of MHCC97L expressed miR-497 was significantly improved than that of MHCC97L with miR-Zip.The expressions of RIPK1 mRNA and NF-κB mRNA were increased in cells with miR-497;The expression of RIPK1 and NF-κB protein were up-regulated in the cells with miR-497.Conclusion:The miR-497 promotes the proliferation and migration of the MHCC97L cell via regulating RIPK1-NF-κB pathway.