柴胡多组分协同治疗乳腺癌作用机制的网络药理学研究

Network pharmacology study on mechanism of radix bupleuri multi-component synergistic therapy for breast cancer

目的:运用网络药理学方法筛选柴胡治疗乳腺癌的有效成分和作用靶点,探究其成分-靶点-疾病的关系。方法:通过中药系统药理学数据库信息挖掘及文献检索收集筛选柴胡的活性成分;多个数据库联用筛选柴胡的作用靶点及乳腺癌疾病相关基因,构建成分靶点-疾病靶点的可视化网络拓扑关系图,筛选出核心靶点。进一步进行蛋白互作网络分析、GO生物学过程富集、信号通路富集分析。结果:柴胡的11个活性成分可通过调控PTGS2、NOS2、AR、ESR2等52个乳腺癌疾病靶点,影响类固醇激素受体活性等生物学过程及内分泌抵抗、铂耐药、乳腺癌相关信号通路,从而发挥治疗乳腺癌的作用。结论:本研究初步验证了柴胡治疗乳腺癌的靶点及作用方式,可为进一步揭示其作用机制奠定基础。

Objective:To explore the mechanism of Radix Bupleuri Treatment of breast cancer through network pharmacology.Methods:TCMSP Database was used and related literature was collected to screen out the active constituents of Radix Bupleuri.Multiple databases were used to search the targets of the constituents and the disease.Next,a visual map of the compound-target-path network was constructed.The protein-protein interaction network was visually analyzed.Finally,the enrichment analysis of GO biological process and pathway enrichment analysis were carried out.Results:Active compounds of Radix Bupleuri related to disease targets have been obtained,and they have played therapeutic roles for breast cancer through mainly regulating target proteins such as PTGS2,NOS2,AR and ESR2.Meanwhile,the active compounds of Radix Bupleuri have an impact on biological processes such as steroid receptor activity and endocrine resistance,platinum resistance,breast cancer-related signaling pathways.Hence,it plays a role in the treatment of breast cancer.Conclusion:The results of this study preliminarily validates the target and mode of Radix Bupleuri in the treatment of breast cancer,and lay a foundation for further revealing its mechanism of action.