维生素E缓解蛋白质氧化豆粕对蛋鸡生产性能、消化功能和鸡蛋品质的影响

The protective effects of vitamin E on laying performance, digestive function and egg quality in laying hens fed with protein-oxidized soybean meal

[目的]本试验旨在研究采食蛋白质氧化豆粕对蛋鸡生产性能、消化功能和鸡蛋品质的影响及维生素E(VE)的缓解作用。[方法]将384只41周龄健康的海兰褐蛋鸡随机分为4组,每组8个重复,每个重复12只蛋鸡。对照组蛋鸡饲喂用冷冻保鲜豆粕配制的基础日粮,蛋白质氧化组蛋鸡饲喂用蛋白质氧化豆粕配制的日粮(豆粕常温储存30 d),以及分别在蛋白质氧化组的基础上添加100 mg·kg^-1合成VE(合成VE组)和天然VE(天然VE组)的试验日粮,预试1周,试验期为6周。[结果]与蛋白质氧化组相比,添加天然VE显著提高蛋鸡的产蛋率并显著降低蛋鸡的料蛋比(P<0.05);与对照组和蛋白质氧化组相比,添加合成VE显著降低蛋鸡的采食量(P<0.05)。与蛋白质氧化组相比,添加合成或天然VE可显著提高蛋鸡血清中高密度脂蛋白胆固醇浓度(P<0.05);与对照组和蛋白质氧化组相比,合成VE显著降低蛋鸡血清中低密度脂蛋白胆固醇浓度(P<0.05)。与对照组相比,蛋白质氧化豆粕显著降低蛋鸡回肠食糜中的胰蛋白酶活性(P<0.05),添加天然VE后恢复至对照组水平(P>0.05),添加合成和天然VE均显著提高蛋鸡空肠食糜中的胰蛋白酶活性(P<0.05);与蛋白质氧化组相比,合成VE显著提高蛋鸡胰腺淀粉酶活性(P<0.05)。与对照组及蛋白质氧化组相比,添加合成VE显著提高鸡蛋的蛋白高度和哈夫单位(P<0.05)。[结论]日粮中添加VE可提高摄食蛋白质氧化豆粕日粮蛋鸡的生产性能、消化功能和鸡蛋品质。

[Objectives]This experiment aimed to study the effects of vitamin E(VE)supplementation on laying performance,digestive function,and egg quality of laying hens fed with protein-oxidized soybean meal.[Methods]A total of 384 Hy-Line laying hens(41 weeks of age)were randomly divided into 4 groups with 8 replicates of 12 hens each.The hens in the control group were fed a basal diet with refrigerated fresh-keeping soybean meal,while the hens in the protein-oxidized group were given protein-oxidized soybean meal diet(soybean meal was stored at room temperature for thirty days),and the stored soybean meal diet supplemented with 100 mg·kg^-1 either synthetic VE(synthetic VE group)or natural VE(natural VE group),for 6 weeks,respectively,after a 1-week period of pretest.[Results]Compared with the protein-oxidized group,natural VE inclusion significantly increased laying rate(P<0.05),whereas reduced feed-to-egg ratio of laying hens(P<0.05);compared with the control and protein-oxidized groups,synthetic VE supplementation significantly reduced feed intake(P<0.05).Compared with the protein-oxidized group,inclusion of natural or synthetic VE significantly increased the high-density lipoprotein cholesterol concentration in the serum of laying hens(P<0.05);compared with the control and protein-oxidized groups,synthetic VE supplementation significantly reduced low-density lipoprotein cholesterol concentration in the serum(P<0.05).Compared with the control group,protein-oxidized soybean meal significantly reduced the trypsin activity in the ileal chyme(P<0.05),with it being recovered to normal level by natural VE supplementation(P>0.05);compared with the protein-oxidized group,inclusion of natural and synthetic VE both significantly increased(P<0.05)trypsin activity in the jejunal chyme of laying hens;compared with the protein-oxidized group,synthetic VE significantly increased pancreatic amylase activity(P<0.05).Compared with the control and protein-oxidized groups,synthetic VE supplementation significantly increased protein height and haugh units in the egg of laying hens(P<0.05).[Conclusions]Dietary VE supplementation could improve laying performance,digestive function,and egg quality of laying hens receiving protein-oxidized soybean meal.