纳米Au修饰煤基活性炭固载葡萄糖氧化酶生物传感器的研究

Construction of glucose oxidase biosensors immobilized by Au-modified coal-based activated carbon

采用置换法将电沉积在煤基活性炭上的Co粒子全部置换为40~100 nm之间的纳米Au,并物理吸附葡萄糖氧化酶(GOx)用作生物传感器。利用SEM、EDS、UV-Vis对Au修饰的活性炭表面形貌及其上活力100 units/mg的GOx固载效果进行了表征,利用计时安培法和循环伏安法测试了电极的电化学性能。结果表明,纳米Au对GOx的吸附提高了GOx在载体上的稳定性,同时提高了GOx活性位点和电极之间的电子传输速率。该传感器对葡萄糖选择性能好,0.05 mol/L的尿酸和抗坏血酸干扰因素未引起显著的电流反应。

Co particles electrodeposited onto coal based activated carbon are all replaced into Au nano-particles with a size of 40-100 nm.Through physical absorption method,glucose oxidase(GOx)is immobilized stably onto the coalbased activated carbon electrode modified with Au nano-particles,which is used as biosensor.The surface topography of Au-modified activated carbon electrode and GOx immobilization effect with a vitality of 100 units·mg-1 are characterized by means of SEM,EDS and UV-Vis.Electrochemical performance of the electrode is tested by chronoamperometry and cyclic voltammetry method.It is shown that the adsorption of GOx by nano-Au enhances the stability of GOx and increases electron transfer rate between the active centers of GOx and electrodes.This biosensor exhibits a good selectivity to glucose,while 0.05 mol·L-1 uric acid and ascorbic acid do not cause its significant current response.