槲皮素对牙本质抗酸蚀性能的影响

Effects of quercetin on the dentin resistance to erosion

目的评价槲皮素对人牙本质抗酸蚀性能的影响,为探寻理想的牙侵蚀症预防和治疗方案提供依据。方法选择福建医科大学附属口腔医院口腔颌面外科门诊提供的因正畸需要而拔除的人第三磨牙50颗,制备牙本质试件128个,用随机数字表法将试件随机分为8组(每组16个)。各组(每组选12个试件)分别用去离子水(对照组)、无水乙醇(对照组)、12.300 mg/L氟化钠、0.120 mg/L氯己定、0.183 mg/L表没食子儿茶素没食子酸脂(epigallocatechin gallate,EGCG)和0.075、0.150、0.300 mg/L槲皮素进行处理。每天37℃下用各组相应试剂浸泡试件2 min、去离子水冲洗并静置于人工唾液2 h后,使用柠檬酸(pH=2.45)进行4次酸蚀循环;连续7 d。分别检测试件酸蚀前后的表面显微硬度及表面轮廓(计算表面硬组织丧失量),用扫描电镜观察试件表面形貌。各组剩余4个试件用10%磷酸脱矿后干燥过夜,37℃下用各组相应试剂浸泡2 min,置于人工唾液中浸提7 d,检测浸提液中Ⅰ型胶原吡啶交联终肽(cross-linked carboxyterminal telopeptide of typeⅠcollagen,ICTP)含量。结果相比对照组,氯己定、槲皮素、EGCG均可显著抑制酸蚀循环引起的牙本质表面软化和硬组织丧失,0.300 mg/L槲皮素组试件酸蚀后表面显微硬度减小率最小[(8.75±4.95)%],表面硬组织丧失量最小[(2.26±1.16)μm],ICTP含量最低[(5.72±0.88)ng],均显著低于氯己定及EGCG组(P<0.05)。各槲皮素组表面硬组织丧失量和ICTP含量差异均无统计学意义(P>0.05);0.300 mg/L槲皮素组酸蚀后表面显微硬度减小率均显著小于0.075和0.150 mg/L槲皮素组(P<0.05)。结论槲皮素可显著提高人牙本质抗酸蚀性能,其中0.300 mg/L槲皮素的作用最佳。

Objective To evaluate the effects of quercetin on dentin resistance to erosion and provide evidence-based recommendations for the prevention and therapy of dental erosion.Methods One hundred and twenty-eight dentin samples were prepared from 50 extracted human wisdom teeth(collected from Department of Oral Surgery,School and Hospital of Stomatology).Ninety-six samples were randomly divided into 8 groups using the following different soaking solutions:deionized water,ethanol(control groups),12.300 mg/L sodium fluoride,0.120 mg/L chlorhexidine,0.183 mg/L epigallocatechin gallate(EGCG),and 0.075,0.150 and 0.300 mg/L quercetin.In each group,twelve specimen was prepared.Before daily acid challenge,the samples were immersed in the respective solutions for 2 min,rinsed with deionized water,and immersed in artificial saliva for 2 h.The samples were then subjected to 4 cycles of in vitro acid challenges.This protocol was applied for 7 d.The surface microhardness(SMH)and surface profiles were measured before and after erosion using the surface microhardness tester and contact profilometry,respectively.The change in surface profiles and reduction in SMH were used to calculate the substance loss and reduction percentage of SMH(SMH%)respectively.Scanning electron microscope(SEM)images were taken to observe the surface morphology of the samples.Additionally,another thirty two samples were divided into 8 groups(n=4)as mentioned above.The specimens were treated with 10%phosphoric acid and desiccated,immersed in the respective solutions for 2 min,rinsed,and immersed in the artificial saliva at 37℃for 7 d.The content of cross-linked carboxyterminal telopeptide of typeⅠcollagen(ICTP)in the soaking solutions were measure quantitatively.Results Compared with the control groups,the application of chlorhexidine,quercetin,and EGCG were effective in preventing the surface softening and substance loss of human dentin after erosion.More specifically,the specimens treated with 0.300 mg/L quercetin exhibited the lowest SMH%[(8.75±4.95)%],the lowest surface substance loss[(2.26±1.16)μm],and the lowest contents of ICTP in the soaking solution[(5.72±0.88)ng],showing significant differences to the chlorhexidine and EGCG treated samples(P<0.05).No significant differences were found in the substance loss and ICTP contents in the three soaking solutions with different concentrations of quercetin(P>0.05).However,the specimens treated with 0.300 mg/L quercetin exhibited significantly lower SMH%than those treated with the other two concentrations of quercetin(P<0.05).Conclusions Within the limitations of the current study,immersion in the quercetin solution is effective in improving the dentin resistance to erosion by inhibiting the dentinal MMP.Among all the concentrations tested,0.300 mg/L quercetin showed the best performance.