肠小体在猪肠道冠状病毒感染中的研究进展

Research progress in enteroids in porcine enteric coronavirus infection

猪肠道冠状病毒是引起仔猪腹泻的重要致病因素,主要感染小肠绒毛上皮细胞,给养猪业造成了巨大的经济损失。由于缺乏能模拟胃肠道高度复杂生理特性的体外研究模型,猪肠道冠状病毒感染与宿主肠上皮之间相互作用的研究也受到了极大的限制。随着干细胞技术的快速发展,一种能模拟肠道复杂的细胞类型及空间结构的体外模型——肠小体引起了人们的广泛关注。与传统的细胞系相比,肠小体不仅能模拟肠的结构和功能,同时还保留宿主的遗传特性,有望成为研究宿主-肠道病原相互作用的一种理想模型。本文就猪肠道冠状病毒以及肠小体在肠道病原研究中的应用进行综述,以期为猪肠道冠状病毒的基础研究提供新的思路与见解。

Porcine enteric coronavirus, an important agent of diarrhea in piglets, primarily infects the villous epithelia of the small intestine, causing substantial economic losses to the pork industry. The lack of an in vitro model that can recapitulate the highly complex physiological properties of the gastrointestinal tract significantly limits the study of the interactions between porcine enteric coronavirus infection and host intestinal epithelium. With the rapid development of stem cell technology, an in vitro model that can mimic diverse cellular nature and complex structure of the intestine-enteroids, has attracted widespread attention. Compared with conventional cell lines, enteroids not only simulate the structure and function of the intestine, but also retain the genetic characteristics of the host. Enteroids would be expected to be an ideal model for studying the interactions of host-enteric pathogens. This article reviews the recent research progress of porcine enteric coronavirus and the applications of enteroids in enteric pathogens research, in order to provide new insights for the future fundamental research of porcine enteric coronavirus.