狭长孢灵芝液体发酵上清液抗氧化活性研究（英文）

Antioxidant activities of the supernatant of liquid fermentation broth of Ganoderma boninense

狭长孢灵芝Ganoderma boninense是最重要的木生真菌之一,主要分布于热带地区,在马来西亚和印度尼西亚是油棕榈树的严重病原菌。本研究以分离自马来西亚雪兰莪州而榄的油棕榈种植园中,生长于油棕榈活树基部狭长孢灵芝子实体的3株菌株为材料,对其抗氧化活性进行了评估。在液体培养的第2、4、6、8、10和12天分别取菌丝体和发酵液,测定菌丝体生物量及发酵上清液中粗多糖、多酚、黄酮和抗坏血酸的含量,并对发酵上清液的相关抗氧化性能指标进行了分析。3个菌株的发酵上清液均表明狭长孢灵芝具有良好的抗氧化活性,但不同菌株在某些次级代谢产物含量和抗氧化指标上表现出强弱程度及出现时间的差异。狭长孢灵芝的多糖和多酚含量以及铁离子还原能力均优于另外两个重要的药用木生真菌,即灵芝Ganoderma lingzhi和栎生桑黄Sanghuangporus quercicola。本研究表明狭长孢灵芝具有开展人工栽培和进行更多药用研究的价值。

Ganoderma boninense is one of the most important wood-inhabiting fungi mainly distributed in tropical areas and is a serious pathogen of oil palms in Malaysia and Indonesia. In this study, three strains of the fungus isolated from basidiocarps growing on the base of living oil palms in the plantation land of Jeram, Selangor, Malaysia, were evaluated for their antioxidant activity. The mycelia and fermentation broth were sampled on the second, fourth, sixth, eighth, tenth, twelfth and fourteenth days during liquid cultivation. Mycelial biomass and the content of crude polysaccharides, polyphenols, flavonoids and ascorbic acids, as well as eight antioxidant indicators of fermentation broth were determined. The supernatant of the fermentation broth of the three strains all showed significant antioxidant activities, although different strains showed variation in level and appearance time of the content of certain secondary metabolites and antioxidant activities. Our results indicated that G. boninense had higher content of polysaccharides and polyphenols and stronger ferric ion reducing antioxidant power than previously well-studied medicinal Ganoderma lingzhi and Sanghuangporus quercicola. Facts have proved that G. boninense showed valuable for artificial cultivation and further intensive medicinal studies.