甘蔗纤维固定灵芝菌丝及其在液体发酵中的应用

Ganoderma lingzhi mycelia immobilized with sugarcane fiber and its application in liquid fermentation

以甘蔗纤维作为灵芝菌丝固定载体,通过扫描电子显微镜观察确定载体固定时间,通过液体发酵灵芝菌丝球大小形态、生物量、胞外多糖和胞外三萜含量确定载体形状、大小与数量。结果表明,灵芝菌丝在载体上固定时间为7d,载体为1.5cm×1.5cm(直径×高度)的圆柱体小块(Y1.5),接种数量6块,可连续稳定发酵7代。以甘蔗纤维固定发酵制备灵芝液体发酵种子,发酵后菌丝球大小均一,生物量、胞外多糖含量和三萜含量分别提高78%、84%和60%。

Sugarcane fiber was used as the immobilized carrier of Ganoderma lingzhi mycelia. The mycelial immobilized time of the carrier was determined by scanning electron microscope observation, and the carrier shape, size and quantity were determined by mycelial pellet size, biomass, extracellular polysaccharide and extracellular triterpene content of liquid-fermented G. lingzhi. The results showed that inoculation of six pieces of cylindrical-shaped and 1.5 cm×1.5 cm sized(diameter×height)(Y1.5) mycelial carriers undergoing immobilized period of seven days could successively and stably maintain fermentation for seven generations. The liquid fermentation seeds were prepared by fixed fermentation of sugarcane fiber carrier. The size of mycelial pellets was uniform after fermentation, and the biomass, extracellular polysaccharide content and triterpenoid content increased by 78%, 84% and 60%, respectively.