摘要
采用多种色谱技术从九蒸九晒熟地黄70%丙酮提取物中分离得到6个生物碱,根据化合物的理化性质及波谱特征对其结构进行鉴定,分别为地黄新碱A(4-{[(5-O-α-D-galactopyranosyloxy)methyl]-1H-pyrrole-2-carbaldehyde-1-yl}butyric acid methyl ester)(1)、baimantuoluoamide B(2)、capparisine C(3)、harman-3-carboxylic acid(4)、(2S)-1-[2-(furan-2-yl)-2-oxoethyl]-5-oxopyrrolidine-2-carboxylate(5)和1-[2-(furan-2-yl)-2-oxoethyl]pyrrolidin-2-one(6)。其中,化合物1为新化合物,化合物2~6均首次从地黄中分离得到。采用细胞实时监控检测方法(RTCA)探究6个化合物对脂多糖(LPS)诱导的NRK-52e细胞损伤的干预作用,结果表明,化合物1~3对LPS诱导的NRK-52e细胞损伤均具有保护作用。
The chemical constituents of Rehmanniae Radix Preparata were prepared according to the traditional method of"jiu zheng jiu shai"and investigated using multiple chromatographic methods.Six alkaloids were isolated and their structures were elucidated from spectral data and physicochemical properties,as follows:rehmanniae alkaloid A(4-{[(5-O-á-D-galactopyranosyloxy)methyl]-1 H-pyrrole-2-carbaldehyde-1-yl}butyric acidmethyl ester)(1),baimantuoluoamide B(2),capparisine C(3),harman-3-carboxylic acid(4),(2 S)-1-[2-(furan-2-yl)-2-oxoethyl]-5-oxopyrrolidine-2-carboxylate(5),and 1-[2-(furan-2-yl)-2-oxoethyl]pyrrolidin-2-one(6).Among them,compound1 is a new alkaloid.Compounds 2-6 were newly isolated from Rehmannia glutinosa Libosch.The effect of compounds 1-6 on NRK-52 e cell injury induced by LPS was investigated.The results show that compounds 1-3 exhibit protective effects against LPS-induced damage to NRK-52 e cells.
作者
吕锦锦
张靖柯
张贝贝
刘娟娟
李孟
石静亚
朱登辉
杨航
郑晓珂
冯卫生
LU Jin-jin;ZHANG Jing-ke;ZHANG Bei-bei;LIU Juan-juan;LI Meng;SHI Jing-ya;ZHU Deng-hui;YANG Hang;ZHENG Xiao-ke;FENG Wei-sheng(School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China;Co-construction Collaborative Innovation Center for Chinese Medicine and Respiratory Disease Diagnosis by Henan and Education Ministry of P.R.China,Zhengzhou 450046,China)
出处
《药学学报》
CAS
CSCD
北大核心
2020年第2期289-293,共5页
Acta Pharmaceutica Sinica
基金
国家重点研发计划中医药现代化研究重点专项“地黄特色中药材产业链关键技术研究”(2017YFC1702800)