基于荧光光谱的2,4,6-三硝基苯酚(TNP)快速检测体系

Rapid Detection System of 2,4,6-Trinitrophenol(TNP) Based on Fluorescent Probe

2,4,6-三硝基苯酚(TNP)是一种威力强于2,4,6-三硝基甲苯(TNT)的猛炸药,同时染料、医药、皮革等行业中被大量使用,由于TNP强大的爆炸威力和环境毒性,使得近年来TNP的快速检测受到越来越多的关注。该研究通过一步反应合成了TNP荧光探针,利用探针的荧光光谱实现了TNP的快速检测。研究了该荧光探针的荧光特性,探针在水溶液中具有较高的荧光强度,在493 nm激发波长下发射波长为547 nm。荧光探针体系与TNP作用后,其发射光谱有明显的减弱,可能是由于荧光探针与TNP结合后,发生了荧光猝灭而改变了其聚集状态。在实验中,测试了荧光探针体系与不同浓度TNP溶液的作用,荧光光谱分析结果表明TNP对荧光探针体系具有良好的猝灭效果,在20~80μmol·L^-1的范围内,547 nm处的荧光强度I547值与TNP的浓度呈现出良好的线性关系,线性回归方程I547=907521.6-9955c(R2=0.9921),并推算出了检测限为4.55μmol·L^-1。该荧光光谱在探针与TNP的作用1 min后即达到稳定,响应时间短,可以实现快速检测;TNP检测的荧光光谱不受对苯酚(PHE)、2,4-二硝基甲苯(DNT)、2,4,6-三硝基甲苯(TNT)、2,4-二硝基苯酚(DNP)、4-硝基苯酚(NP)等结构类似干扰物和Ca^2+,K^+,Ba^2+,Cl^-,SO■,NO-3常见阴阳离子的干扰。考察了尘土基质对荧光探针荧光光谱的影响,在实验室内制作了添加TNP的模拟爆炸尘土样本,使用上述荧光探针体系进行检测,实验表明尘土对该荧光探针发射光谱的稳定性及其与TNP的相互作用均无干扰,该体系的荧光发射光谱可以用于尘土中TNP的定量分析,具有较好的实际应用前景。

The rapid detection of 2,4,6-trinitrophenol(TNP)has attracted more and more attention in recent years,due to its powerful explosive hazard and environmental toxicity.In this study,TNP fluorescent probe based on perylene imide was synthesized by one-step synthesis,and the rapid detection of TNP was realized by the fluorescent spectra of the probe.The fluorescence characteristics were investigated,and the fluorescence intensity was high in aqueous solution,stimulated at 493 nm at the emission wavelength of 547 nm.The emission spectra of the fluorescent probe system decreased obviously after the interaction with TNP,which might be due to the fluorescence quenching after the combination of the fluorescent probe and TNP,changing the aggregation state.The fluorescence changes of the probe system after the interaction with TNP could also be observed under the 365 nm handheld ultraviolet lamp,and the results could also be judged by naked eye.In the experiment,different concentrations of TNP solution were tested and the fluorescence spectra analysis showed that TNP had good quenching effect on the fluorescent probe system.The fluorescence intensity of the probe system at 547 nm,I547 value,presented a good linear relationship in the concentration of 20~80μmol·L^-1 with the regression equation of I547=907521.6-9955c(R2=0.9921),and the detection limit was calculated to be 4.55μmol·L^-1.The fluorescence spectra of the probe were stable after 1 minute of interaction with TNP and the response time was short.The fluorescence spectra of the probe were not affected by structure-like interfering compounds,such as phenol(PHE),2,4-dinitrotoluene(DNT),2,4,6-trinitrotoluene(TNT),2,4-dinitrophenol(DNP),4-nitrophenol(NP)and common anions and cations(Ca^2+,K^+,Ba^2+,Cl^-,SO■,NO-3).The interference of the dust matrix on fluorescence spectra was also investigated.Simulated explosive dust samples with TNP were made in the laboratory and fluorescence spectra were detected by the fluorescent probe system.The experiment showed that the fluorescence spectra of the probe and the interaction with TNP are not disturbed by dust.The fluorescence emission spectra can be applied to the quantitative analysis of TNP in dust and has good practical application prospects.