脑栓通胶囊对动脉粥样硬化小鼠血脂水平的影响

Influence of Naoshantong Capsule on Blood Lipid Levels in Atherosclerotic Mice

目的观察脑栓通胶囊对动脉粥样硬化小鼠血脂水平的影响,探讨脑栓通胶囊治疗脑卒中的机制。方法6周龄雄性ApoE^-/-小鼠,采用高脂饮食法建立动脉粥样硬化模型,造模成功后随机分为模型组、脑栓通胶囊低剂量组、脑栓通胶囊高剂量组。低剂量组给予脑栓通胶囊0.5 g/kg体质量,高剂量组给予脑栓通胶囊1.0 g/kg体质量,对照组给予等量的蒸馏水。连续给药12周后腹主动脉取血,分离血清。酶偶联比色法测定血清总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、低密度脂蛋白胆固醇(low density lipoprotein-cholesterol,LDL-C)、高密度脂蛋白胆固醇(high density lipoprotein-cholesterol,HDL-C)水平。结果与模型组相比,脑栓通胶囊低剂量组和高剂量组均显著降低血清中TC、TG和LDL-C的含量,但对HDL-C含量无影响。结论脑栓通胶囊可以调节血脂水平,其治疗脑卒中的作用机制很可能与该途径有关。

Objective To observe the effect of Naoshuantong capsule on blood lipid levels in atherosclerotic mice and further explore its related mechanism of treatment on stroke.Method Six weeks old ApoE^-/-mice were fed with western diet for 12 weeks and randomly divided into three group:model group,Naoshuantong low dose group and Naoshuantong high dose group.The high and low dose group were given intragastric administration with 1.0 g/kg and 0.5 g/kg for 12 weeks,respectively.The model group was given normal saline of equal volume distilled water.After 12 weeks administration with Naoshuantong capsule,serum was collected from the abdominal aorta for lipid levels measurement.The levels of serum lipids TC,TG,LDL-C and HDL-C were detected by enzyme method.Result Compared with model group,Naoshuantong capsule low group and high group both could decrease the levels of TC,TG,LDL-C significantly,but there was no influence on HDL-C.Conclusion Naoshuantong capsule could regulate the level of blood lipid,and its mechanism of treating stroke may be related to this pathway.