5周龄至109周龄Wistar大鼠体质量的正常参考值及增长速度

The Normal Reference Value of Body Weight and the Rate of Body Weight Gain in Wistar Rats Aged from 5 to 109 Weeks

目的了解Wistar大鼠在其大部分生命过程中的体质量及其增长速度。方法慢性毒性与致癌合并试验中的对照组,采用4周龄SPF级Wistar大鼠,雌、雄性大鼠各60只,试验前观察1周,常规饲料喂饲;试验期为104周,在试验的前13周内,每周测量一次体质量,之后每4周测量一次体质量。结果Wistar大鼠自5周龄至109周龄生命过程中共37个时点的体质量及其增长速度:(1)随着鼠龄的增长,大鼠体质量持续增长,雄性和雌性分别在94周龄和102周龄达到体质量最大值,分别为(755.7±103.4)g和(515.4±96.6)g,之后体质量逐渐减低。(2)体质量增长速度随鼠龄的增长呈逐渐减慢的趋势,雄性和雌性分别在第66周龄和第82周龄首次出现负增长,负增长的幅度最终变得更大。结论Wistar大鼠5周龄至109周龄共37个时点的体质量正常参考值及其增长速度,可对已有的Wistar大鼠体质量正常值的相关文献资料进行进一步完善和补充,为Wistar大鼠体质量正常值的实验室间比较或相关技术人员提供一些有用的参考。

Objective To learn about the body weight and rate of body weight gain of Wistar rat during most of its life.Method The control group of a combined chronic toxicity and carcinogenicity test included 60 male and 60 female Specific Pathogen-Free Wistar rats(4 weeks old).The rats were acclimated for 1 week prior to initiation of the test.The rats were dieted with conventional feed.The study duration was 104 weeks.Body weight were measured weekly for the first 13 weeks and once every 4 weeks thereafter.Result The body weight and rate of body weight gain of Wistar rats at 37 time points during their life from 5 to 109 weeks were reported:(1)With the increase of age of the rats,the body weight increased continuously;males and females reached their maximum body weight at 94 and 102 weeks,which were(755.7±103.4)g and(515.4±96.6)g,respectively;their body weight decreased thereafter.(2)The rate of body weight gain decreased with the increase of rat age;the negative rates were first observed at 66 and 82 weeks for males and females,respectively;the negative rate became more negative finally.Conclusion The data about the normal reference body weight and the rate of body weight gain of Wistar rats at 37 time points during their life from 5 to 109 weeks reported in this paper may further improve and supplement the existing literature on Wistar rats’normal reference body weight;and provide some useful reference for its inter-laboratory comparison and for the relevant technical staffs.