香烟烟雾染毒改良方法的应用

Application of Improved Cigarette Smoke Exposure Method

目的探讨烟雾染毒的改良方法对小鼠存活率的影响。方法在普通烟雾染毒方法的基础上,改进制作一种可向染毒箱底部通入空气以增加氧气量,并能同时监测温度的染毒装置。将实验对象昆明系小鼠分为对照组(在普通染毒装置中,不染毒),染毒1组(普通染毒方法),染毒2组(改进的染毒方法)进行实验,应用统计软件SPSS20.0分析比较各组之间小鼠的生存率、染毒装置内氧浓度以及总悬浮颗粒浓度变化。结果共选取160只昆明系小鼠,并随机分为对照组40只,染毒1组60只,染毒2组60只,均雌雄各半。实验结果发现,染毒2组小鼠的存活率显著高于染毒1组,差异具有统计学意义(x^2=7.647 P=0.02<0.05),染毒1组装置内的氧浓度明显低于染毒2组氧浓度,且染毒2组装置内的氧浓度波动小,差异具有统计学意义(F=5.260,P=0.045<0.05)。染毒1组和染毒2组装置内的TSP浓度差异没有统计学意义,说明改进的烟雾染毒装置不影响毒物的剂量和染毒过程。结论改良的烟雾染毒装置会使实验动物在非药物因素作用下的死亡率降低,且不影响实验染毒过程。

Objective To investigate the effect of improved method of smoke exposure on survival rate in mice.Method Based on the common smoke exposure method,we have made an infection device which capable introducing air into the bottom of the poisoning box that in order to increase the amount of oxygen and monitor the temperature simultaneously.The Kunming mice(the experimental subjects)were divided into the control group(in the common poisoning device,no poisoning),the poisoning group 1(common poisoning method),the poisoning group 2(the improved poisoning method),and use the statistical software spss20.0 analysis to compare the survival rate,the oxygen concentration in the poisoning device,and the total suspended particle concentration of mice in each group(the control group,poisoning group 1,poisoning group 2).Result There were total of 160 Kunming mice,and divided into the control group(n=40),the poisoning group 1(n=60),and the poisoning group 2(n=60)randomly,both with male and female.The experimental results showed that the survival rate of the mice in the poisoning group 2 was significantly higher than that in poisoning group 1(x^2=7.647 P=0.02<0.05).The oxygen concentration in poisoning group 1 was significantly lower than that in poisoning group 2,and the oxygen concentration fluctuation in the poisoning group 2 was small,and the difference was statistically significant(F=5.260,P=0.045<0.05).There was no statistically significant difference in TSP concentration between poisoning group 1 and poisoning group 2,indicating that the improved smoke exposure device did not affect the dose and exposure process of the poison.Conclusion The improved smoke exposure device will reduce the mortality of experimental animals under non-pharmaceutical factors and will not affect the experimental exposure process.