LncRNA AK093407在结直肠癌中的生物学作用研究

Research on Biological Role of LncRNA AK093407 in Colorectal Cancer

目的:观察LncRNA AK093407在人结直肠癌中的生物学作用。方法:qRT-PCR检测LncRNA AK093407在人结直肠癌组织及结直肠癌细胞HCT-116中的表达水平;siRNA沉默LncRNA AK093407在结直肠癌细胞中的表达:MTT实验检测细胞增殖;AnnexinV/PI染色法检测细胞凋亡;PI染色法检测细胞周期;western blot检测细胞凋亡相关蛋白Caspase3、Caspase9、Caspase10、Bax、Bcl-2,细胞周期相关蛋白P21、P27,及p-STAT3的表达。结果:LncRNA AK093407在人结直肠癌组织中的表达量高于癌旁组织;LncRNA AK093407在人结直肠癌细胞HCT-116中的表达量高于正常结直肠上皮细胞NM460;沉默LncRNA AK093407后,HCT-116细胞增殖活性降低,细胞凋亡增强,细胞周期阻滞于G1/S期;细胞凋亡相关蛋白Caspase3、Caspase9、Caspase10、Bax表达增多,Bcl-2表达降低,细胞周期相关蛋白P21、P27表达增加,p-STAT3表达降低。结论:LncRNA AK093407在结直肠癌组织及HCT-116细胞中高表达,沉默LncRNA AK093407后可抑制细胞增殖、诱导细胞凋亡及细胞周期阻滞。

Objective: To study the biological role of LncRNA AK093407 in human colorectal cancer cells.Methods:QRT PCR was used to detect the expression of lncRNA AK093407 in human colorectal cancer and HCT-116 cells;siRNA silenced the expression of lncRNA AK093407 in colorectal cancer cells:MTT assay was used to detect cell proliferation;annexin V/PI staining was used to detect cell apoptosis;PI staining was used to detect cell cycle;Western staining was used to detect cell cycle.The expression of Caspase-3,caspase-9,caspase-10,Bax,Bcl-2,p21,p27 and p-STAT3 were detected by blot.Results:The expression of lncRNA AK093407 in human colorectal cancer was higher than that in adjacent tissues;the expression of lncRNA AK093407 in HCT-116 was higher than that in NM460;lncRNA was silenced After AK093407,the proliferation activity of HCT-116 cells decreased,apoptosis increased,cell cycle blocked in G1/S phase;the expression of caspase 3,caspase 9,caspase 10,Bax,Bcl-2,p21,p27 and p-STAT3 decreased.Conclusion:LncRNA AK093407 is highly expressed in colorectal cancer and HCT-116 cells.Silencing lncRNA AK093407 can inhibit cell proliferation,induce cell apoptosis and cell cycle arrest.