血清MicroRNA 122和MicroRNA-130a水平在隐匿性乙型肝炎病毒感染中的诊断价值初探

Diagnostic Value of Serum MicroRNA 122 and Microrna-130a Levels in Occult Hepatitis B Virus Infection

目的:分析血清微小RNA-122(miR-122)与miR-130a在隐匿性乙型肝炎(OBI)中相关性,探讨其在OBI的诊断中的作用。方法:选取OBI患者60例(OBI组)、非活动性HBsAg携带患者(ASC)60例(ASC组)、慢性乙型肝炎(CHB)患者60例(CHB组)及可疑患者60例(对照组),均采用实时荧光定量方法检测血清miR-122、miR-130a含量。结果:OBI组miR-122相对含量明显低于ASC组、对照组及CHB组(均P<0.05),miR-130a相对含量明显高于ASC组、对照组及CHB组(均P<0.05);且miR-122相对含量在OBI组、对照组、ASC组、CHB组间依次增高(均P<0.05),miR-130a相对含量在OBI组、CHB组、ASC组、对照组间依次降低(均P<0.05)。OBI组miR-130a/miR-122值明显高于对照组、ASC组及CHB组(均P<0.05)。根据Pearson相关性研究显示,OBI组miR-122与miR-130a水平呈负相关(r=0.421,P<0.05)。ROC曲线分析显示,血清miR-122、miR-130a及miR-130a/miR-122值区分OBI与可疑患者的曲线下面积为0.859(95%CI:0.762~0.955)、0.844(95%CI:0.739~0.950)、0.945(95%CI:0.018~0.989),区分OBI与ASC的曲线下面积是0.942(95%CI:0.021~0.995)、0.813(95%CI:0.699~0.927)、0.964(95%CI:0.012~0.997),区分OBI与CHB的曲线下面积是0.998(95%CI:0.000~1.000)、0.646(95%CI:0.503~0.789)、0.993(95%CI:0.000~1.000)。结论:血清miR-122含量降低、miR-130a含量增高和OBI有关联,对OBI有重要诊断价值,miR-130a/miR-122值的诊断价值高于血清miR-122及miR-130a的单独诊断价值。

Objective:To study the correlation between serum microRNA-122(miR-122)and miR-130a and occult hepatitis B(OBI),and to explore their diagnostic value for OBI.Methods:60 patients with OBI(OBI group),60 carriers of inactive HBsAg(ASC)(ASC group),60 patients with chronic hepatitis B(CHB)(CHB group)and 60 suspicious patients(control group)were selected.The expression of serum miR-122 and miR-130a was detected by real-time fluorescence quantitative method.Results:The relative expression level of serum miR-122 in OBI group was significantly lower than that in the other three groups(all P<0.05).However,the relative expression level of miR-130a was significantly higher than that in the three groups(all P<0.05).The relative expression level of serum miR-122 in OBI group,the control group,ASC group and CHB group increased in turn(all P<0.05),and the relative expression level of miR-130a in OBI group,CHB group,ASC group and control group decreased in turn(all P<0.05).The value of miR-130a/miR-122 in OBI group was significantly higher than that in the other three groups(all P<0.05).Pearson correlation analysis showed that the expression of serum miR-122 was negatively correlated with the expression of miR-130a in patients with OBI(r=0.421,P<0.05).ROC curve analysis showed that the areas under the curves of serum miR-122,miR-130a and miR-130a/miR-122 value for differentiating patients with OBI and suspicious patients were 0.859(95%CI:0.762~0.955),0.844(95%CI:0.739~0.950)and 0.945(95%CI:0.018~0.989),respectively.The areas under the curves for differentiating OBI and ASC were 0.942(95%CI:0.021~0.995),0.813(95%CI:0.699~0.927)and 0.964(95%CI:0.012~0.997),respectively.The areas under the curves for differentiating OBI and CHB were 0.998(95%CI:0.000~1.000),0.646(95%CI:0.503~0.789)and 0.993(95%CI:0.000~1.000),respectively.Conclusion:The decreased expression of serum miR-122 and the increased expression of miR-130a are associated with OBI,which is of important diagnostic value for OBI.The diagnostic value of miR-130a/miR-122 is higher than that of serum miR-122 and miR-130a alone.