哺乳期饲喂白藜芦醇和地衣芽孢杆菌对0-2月龄湖羊生长性能、营养物质消化代谢和血清指标的影响

Growth Performance, Nutrient Digestibility and Serum Parameters in 0-2 Months Old Hu Lambs

【目的】从羔羊生长性能、营养物质消化代谢、血清抗氧化和免疫指标方面研究白藜芦醇(Resveratrol)和地衣芽孢杆菌(Bacillus licheniformis)对0-2月龄羔羊的应用效果。【方法】选取体重为(3.82±0.46)kg的新生湖羊羔羊120只,分为4个处理组:正常断奶组(CON组,49 d断奶)、早期断奶组(EW组,21 d断奶)、白藜芦醇组(RSV组,饲喂10 mg·kg-1 BW白藜芦醇,21 d断奶)和地衣芽孢杆菌组(BL组,饲喂60 mg·kg-1 BW地衣芽孢杆菌,活菌数≥2×109cfu/g,21 d断奶)。每个处理3个重复,每个重复10只羔羊。从4日龄开始,RSV组和BL组分别通过口腔灌服白藜芦醇和地衣芽孢杆菌。羔羊于56-63日龄期间进行消化代谢试验,并在63日龄时采集血液样品。试验期共63 d。【结果】羔羊21 d体重(BW)和0-21日龄开食料采食量及平均日增重(ADG)在各处理组之间差异不显著(P>0.05)。EW组、RSV组和BL组羔羊的49 d和63 d体重均显著低于CON组(P<0.01),开食料采食量在21-49日龄期间显著升增加(P<0.01)。试验全期(0-63 d),EW组、RSV组和BL组羔羊ADG显著低于CON组(P<0.01),分别降低了18.5%、15.1%和20.5%。CON组和EW组羔羊干物质(DM)和有机物(OM)的消化率显著高于RSV组(P<0.05),并且EW组OM消化率显著高于RSV组(P<0.05)。CP、EE、Ca、P的表观消化率在各组之间无显著性差异(P>0.05)。EW组NDF和ADF的消化率显著高于RSV组(P<0.05),其他各组之间NDF和ADF的消化率无显著性差异(P>0.05)。不同处理对羔羊血清抗氧化、免疫及炎症等指标均无显著性影响(P>0.05)。【结论】21日龄断母乳直接转为采食固体饲料的饲喂方式,会造成羔羊生长受阻,早期饲喂地衣芽孢杆菌和白藜芦醇对湖羊羔羊生长性能、营养物质消化代谢、血清抗氧化和免疫指标等方面无明显作用。

【Objective】 The study was conducted to evaluate the effects of Resveratrol and Bacillus licheniformis on growthperformance, nutrient digestibility and serological parameters in lambs.【Method】A total of 120 neonatal Hu lambs((3.82±0.46) kg of BW) randomly divided into four groups, including CON group(lambs were conventional weaned at 49 days of age), EW group(lambs were early weaned at 21 days of age), RSV group(lambs were supplemented with 10 mg·kg-1 BW Resveratrol and early weaned) and BL group(lambs were supplemented with 60 mg·kg-1 BW Bacillus licheniformis with ≥4.0× 109 CFU/g live bacteria and early weaned). Each group had 3 replicates with 10 lambs in each replicate. The digestion trial was carried out during day 56-63, and serum parameters were determined on day 63. The experiment lasted 63 d.【Result】There were no differences in body weight(BW) at day 21, average daily gain(ADG) and solid feed intake during day 1 to day 21 between the groups(P>0.05). The BW of day 21 and day 49 were significantly lower(P<0.01) but solid feed intake of day 21-49 were significantly higher(P<0.01) in EW, RSV and BL groups than that in CON group. During the whole experiment, the ADG of ER, RSV, and BL groups were significantly lower than that of CON group(P<0.01), which were relatively decreased by 18.5%, 15.1% and 20.5%, respectively. The digestibility of dry matter(DM) and organic matter(OM) were significantly higher in CON and EW group than that in RSV group(P<0.05), and the OM digestibility was significantly higher in EW group than that in RSV group(P<0.05). The apparent digestibility of CP, EE, Ca and P were not significantly different among the groups(P>0.05). The digestibility of NDF and ADF were significantly higher in EW group than that in RSV group(P<0.05), but there was no significant difference between the other groups(P>0.05). There were no significant differences in serum anti-oxidation, immunity and inflammation parameters of lambs between groups(P>0.05).【Conclusion】 The results showed that weaning at 21 days of age and directly switching them to solid feed had negative effects on lambs’ growth performance with the BW reducing by about 15% at 63 days of age. The supplementation of Resveratrol and Bacillus licheniformis seemed no influence on growth performance, nutrient digestibility, serum antioxidants and immunity parameters in Hu lambs.