平板式光生物反应器中光照强度对极大螺旋藻生长和生理生化的影响

Effects of light intensity on the growth,physiological and biochemistry parameters of Spirulina maxima in a flat plate photobioreactor

以极大螺旋藻(Spirulina maxima)为材料,采用平板式光生物反应器在不同光照强度下(278、327、373、420和463μmol·m-2·s-1)对其进行培养。通过测定藻生物量、生长速率、叶绿素a、类胡萝卜素、藻蓝素、可溶性蛋白和可溶性糖的含量探讨光照强度对极大螺旋藻生长和生理生化特征的影响。结果表明,在光照强度为373μmol·m-2·s-1时螺旋藻生长最快,生长速率为0.203,培养10 d后生物量达0.298 g·L-1,是初始生物量的6.2倍。极端光强463μmol·m-2·s-1时藻细胞出现死亡。单位质量藻体中叶绿素a、胞内多糖和胞外多糖积累最大值的光强与生长最佳光强相同,即在373μmol·m-2·s-1时产量均最高。类胡萝卜素在高光强420μmol·m-2·s-1时产量最高,而藻蓝素和可溶性蛋白在较低光强278μmol·m-2·s-1时含量显著高于其他各处理组。该研究结果可为平板式光生物反应器在螺旋藻规模化培养中的推广提供理论基础。

In this study,the effect of light intensity on the biomass and high valued additives of Spirulina maxima was explored,which was cultured in the light intensity of 278-463μmol·m-2·s-1 for 10 days in a flat plate photobioreactor.The effects were investigated by determining its biomass,growth rate as well as the contents of chlorophyll a,carotenoid,phycocyanin,soluble protein and soluble sugar.Results showed that the growth rate was the maximum when the light intensity was 373μmol·m-2·s-1.The relative growth rate was 0.203 g·L-1,and the biomass reached to 0.298 g·L-1 after cultured 10 days,6.2 times of the inital biomass.When the light intensity was extremely high,of 463μmol·m-2·s-1,the chlorophyll was oxidized and photolyzed,causing the algae becoming yellow even death.The trends of chlorophyll a,intracellular and extracellular polysaccharide contents were in accordance with the growth rate,and the maximum values were presented at 373μmol·m-2·s-1.The carotenoid content was increased with the increase of light intensity,reaching the highest at 420μmol·m-2·s-1,while the phycocyanin and soluble protein were easy to accumulate at lower light intensity of 278μmol·m-2·s-1.This research might provide some theory bases for scale cultivation of S.maxima using the flat plate photobioreactor.