IL-23受体和IL-17单核苷酸多态性与汉族早产儿坏死性小肠结肠炎的关系

Association of interleukin-23 receptor and interleukin-17 single nucleotide polymorphisms with necrotizing enterocolitis in Chinese Han preterm infants

目的探讨白细胞介素-23受体(IL-23R)基因rs10889677位点、IL-17A基因rs2275913位点和IL-17F基因rs763780位点单核苷酸多态性(SNP)与汉族早产儿坏死性小肠结肠炎(NEC)的关系。方法前瞻性选取2017年1月至2019年1月新生儿重症监护病房收治的100例汉族NEC早产儿为研究对象,其中Ⅱ期63例,Ⅲ期37例;另选取与NEC患儿胎龄、性别匹配的100例早产儿作为对照。采用PCR法和Sanger测序法鉴定rs10889677、rs2275913、rs763780位点的SNP。采用非条件logistic回归分析基因多态性与NEC易感性和病情严重程度的关系。结果rs10889677位点、rs2275913位点基因型和等位基因频率对NEC发病无影响(P>0.05);rs763780位点基因型对NEC发病无影响(P>0.05),但C等位基因携带者相对于T等位基因携带者的NEC发病风险为1.652倍(95%CI:1.052~2.695,P<0.05)。TC+CC基因携带者相对于TT基因携带者的NEC发病风险为1.856倍(95%CI:1.045~3.201,P<0.05)。TC+CC基因携带者相对于TT基因携带者的NECⅢ期的发生风险为2.965倍(95%CI:1.052~6.330,P<0.05);C等位基因携带者相对于T等位基因携带者的NECⅢ期的发生风险为2.363倍(95%CI:1.034~4.093,P<0.05)。结论IL-23R基因rs10889677位点和IL-17A基因rs2275913位点SNP与汉族早产儿的NEC易感性无关,IL-17F基因rs763780位点TC+CC基因型和C等位基因与NEC易感性和NEC病情严重程度有关。

Objective To study the association of single nucleotide polymorphisms(SNPs)of interleukin-23 receptor(IL-23 R)rs10889677,interleukin-17 A(IL-17 A)rs227591,and interleukin-17 F(IL-17 F)rs763780 with necrotizing enterocolitis(NEC)in Chinese Han preterm infants.Methods A total of 100 Chinese Han preterm infants with NEC who were admitted to the neonatal intensive care unit from January 2017 to January 2019 were prospectively enrolled.Of the 100 preterm infants,63 had stage II NEC and 37 had stage III NEC.A total of 100 preterm infants,matched for age and sex,were selected as the control group.PCR and Sanger sequencing were used to determine the SNPs of rs10889677,rs2275913,and rs763780.An unconditional logistic regression analysis was used to investigate the association of SNPs with NEC susceptibility and severity.Results The genotype and allele frequencies of rs10889677 and rs2275913 had no influence on the development of NEC(P>0.05).The genotype of rs763780 had no influence on the development of NEC(P>0.05),but the risk of NEC in the infants carrying C allele was 1.652 times that in those carrying T allele(95%CI:1.052-2.695,P<0.05).The risk of NEC in the infants carrying TC+CC genotype was 1.856 times that in those carrying TT genotype(95%CI:1.045-3.201,P<0.05).The risk of stage III NEC in the infants carrying TC+CC genotype was 2.965 times that in those carrying TT genotype(95%CI:1.052-6.330,P<0.05).The risk of stage III NEC in the infants carrying C allele was 2.363 times that in those carrying T allele(95%CI:1.034-4.093,P<0.05).Conclusions The SNPs of IL-23 R rs10889677 and IL-17 A rs2275913 are not associated with the susceptibility to NEC in Chinese Han preterm infants,while TC+CC genotype and C allele of IL-17 F rs763780 are associated with the susceptibility to NEC and the severity of NEC.