海七鳃鳗(Petromyzon marinus)anoctaimin-1蛋白的生物信息学分析

Bioinformatics Analysis of anoctamin-1 Protein of Petromyzon marinus(Sea lampery)

七鳃鳗是现存的最原始的无颌类脊椎动物之一,也是连接无脊椎动物与脊椎动物的重要环节,对生物的起源与进化有很高的研究价值。anoctamin-1蛋白(ANO1)是一种重要的跨膜蛋白,与细胞内阴离子的跨膜运输相关。以海七鳃鳗为例,利用不同软件对海七鳃鳗ANO1蛋白的理化性质、结构域、蛋白结构特征、物种进化保守性以及系统进化关系进行生物信息学分析表明:海七鳃鳗ANO1的开放阅读框为2 373 bp,编码791个氨基酸,属于anoctamin蛋白家族,具有7个跨膜区;二级结构含有无规则卷曲、α螺旋和β折叠。将海七鳃鳗与其他物种的ANO1氨基酸序列进行同源比对,并构建系统进化树,以确认海七鳃鳗ANO1基因的保守性和进化地位。对ANO1基因及蛋白的生物信息学分析为ANO1基因及蛋白的相关研究提供了重要的信息基础。

Lamprey is one of the most primitive non maxillary vertebrates,and is also an important link to connect invertebrates and vertebrates.It has high research value on the origin and evolution of biology.Anoctamin-1(ANO1) is an important transmembrane protein,which is related to transmembrane transport of intracellular anions.To analyze the ANO1 nucleic and amino acids sequence,the physical and chemical properties,structure domain,protein structure characteristics,conservation of species evolution and phylogenetic relationship were predicted by different software.The open reading frame of sea lamprey ANO1 is 2 373 bp,which encodes 791 amino acids,belonging to the anoctamin protein family and has 7 transmembrane regions.The secondary structure contains random coil,alpha helix and beta turn.The ANO1 amino acid sequences of the sea lamprey and other species were compared and the phylogenetic tree was constructed to confirm the conservatism and evolutionary status of the sea lamprey ANO1 gene.The bioinformation analysis of ANO1 gene and protein provides valuable information for the research of ANO1.