摘要
大肠杆菌(Escherichia coli)、化脓隐秘杆菌(Trueperella pyogenes,Arcanobacterium pyogenes)和坏死梭杆菌(Fusobacterium necrophorum)是与子宫内膜病变相关的主要病原体。本研究使用聚合酶链式反应(PCR)直接鉴定四川地区139例孕妇宫颈分泌物中的病原体,而不进行细菌培养。此外,对上述3种病原体的耐药性进行了考察。结果显示,第1次生产后检查发现子宫内膜炎的发生率(35.25%)高于第2次生产后检查(15.53%)。在6个样品中检测到大肠杆菌,在11个样品中检测到化脓隐秘杆菌,并在10个样品中检测到坏死梭杆菌。在第1次检查中,化脓隐秘杆菌和坏死梭杆菌的子宫污染感染高于第2次检查。药敏实验结果显示,大肠杆菌、化脓隐秘杆菌和坏死梭杆菌分别对9种、6种和7种药物产生耐药性。总之,本研究建立了用于诊断孕妇产后子宫内膜炎的3种主要病原体的标准PCR,该方法更简单、成本低和快捷,且对主要细菌检测具有较高的灵敏度。此外,大肠杆菌的耐药性高于另外2个菌种。
Escherichia coli,Trueperella pyogenes and Fusobacterium necrophorum are the main pathogens associated with endometrial lesions.In this study,polymerase chain reaction(PCR) was used to directly identify pathogens in cervical secretions of 139 pregnant women in Sichuan,without bacterial culture.In addition,the resistance to these three pathogens was examined.The results showed that after the first post-production examination,the incidence of endometritis(35.25%) was higher than the second post-production examination(15.53%).Escherichia coli was detected in 6 samples,Trueperella pyogenes was detected in 11 samples,and Fusobacterium necrophorum was detected in 10 samples.In the first examination,uterine contamination of Trueperella pyogenes and Fusobacterium necrophorum necrosis was higher than the second examination.The results of drug susceptibility testing showed that Escherichia coli,Trueperella pyogenes and Fusobacterium necrophorum were resistant to 9,6 and 7 drugs,respectively.In conclusion,this study established a standard PCR for the diagnosis of three major pathogens of postpartum endometritis in pregnant women.This method is simpler,cheaper,and faster,and has a higher sensitivity for detection of major bacteria.In addition,Escherichia coli is more resistant than the other two bacteria.
作者
彭曦
Peng Xi(Sichuan Normal University Hospital,Chengdu,610068)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2020年第1期454-458,共5页
Genomics and Applied Biology
关键词
孕妇
子宫内膜炎
致病菌
检测
耐药性
PCR
Dairy cow
Endometritis
Pathogenic bacteria
Detection
Drug resistance
PCR