摘要
在单因素实验的基础上,以舌状蜈蚣藻多糖得率为指标,选择料液比、温度和时间进行响应面实验,确定最佳工艺条件,同时测定舌状蜈蚣藻多糖对1,1-二苯基-2-三硝基苯肼(DPPH)自由基以及羟自由基(·OH)的清除能力。结果显示,舌状蜈蚣藻多糖的最佳提取工艺为料液比1∶37 g/mL,提取温度100℃,提取时间4 h,此时的多糖得率为15.23%。舌状蜈蚣藻多糖清除DPPH自由基和羟自由基的半抑制质量浓度(IC50)分别为12.61 mg/mL和2.05 mg/mL,具有较好的体外抗氧化活性。
The extraction of polysaccharides from Grateloupia livida(GLP)was optimized using one-factor-at-a-time method and response surface methodology.Solid-to-solvent ratio, temperature and time were used as independent variables while polysaccharide yeild was used as response variable.The antioxidant activity of GLP was investigated by measuring scavenging activities against 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH) and hydroxyl radicals(·OH).The results showed that the optimum extraction conditions were found to be extraction at 100 ℃ for 4 h with a solid-to-solvent ratio of 1∶37 g/mL, while polysaccharide yeild was 15.23%. GLP possessed strong antioxidant activity with half inhibitory concentration(IC50) for scavenging of DPPH and hydroxyl radicals. 12.61 mg/mL and 2.05 mg/mL,respectively.
作者
陈胜军
刘欢
杨少玲
杨贤庆
戚勃
胡晓
CHEN Shengjun;LIU Huan;YANG Shaoling;YANG Xianqing;QI Bo;HU Xiao(National Research and Development Center for Aquatic Product Processings Key Laboratory of Aquatic Product Processing,Ministry of Agriculture and Rural Affairs,South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510300,Guangdong,China;Co-Innovation Center o f Jiangsu Marine Bio-industry Technology y Lianyungang222005,Jiangsu,China;College of Food Science&Technology.Shanghai Ocean University,Shanghai 201306,China)
出处
《上海海洋大学学报》
CAS
CSCD
北大核心
2020年第1期153-160,共8页
Journal of Shanghai Ocean University
基金
中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金(2017ZD03)现代农业产业技术体系专项资金(CARS-50)农业部财政重大专项(NFZX2013)广东省海洋与渔业发展专项资金(2017A0001)。
关键词
舌状蜈蚣藻
多糖
响应面
抗氧化活性
Grateloupia livida
polysaccharides
response surface methodology
antioxidant activity
