Tomm34在卵巢癌中的表达及其与癌症分期的关系

Expression of Tomm34 in ovarian cancer and its relationship with tumor staging

目的研究线粒体外膜转运子34(Tomm34)在卵巢癌组织中的表达,探讨其表达水平与癌症分期的关系。方法收集2016年2月-2018年10月在深圳市南山区人民医院诊断为卵巢癌并接受手术治疗的94例患者资料纳入本次研究。采用免疫组织化学法检测肿瘤中Tomm34的表达情况。比较患者的临床特征和组织学特征与Tomm34表达水平的关系。结果所有样本进行Tomm34 IHC实验后,有11例样品(11.70%)为0分,29例样品(30.85%)为1分,32例样品(34.04%)为2分,22例样品(23.40%)为3分。两种病理分类样本的Tomm34水平比较,1类样品的Tomm34表达评分(1.49±0.92)显著低于2类样品(1.91±0.97),差异具有统计学意义(P<0.05)。与野生型p53基因的样品比较,携带突变型p53基因的卵巢癌样本的Tomm34表达评分显著更高(P<0.05)。不同国际妇产科联合会(FIGO)分期卵巢癌样本的Tomm34表达评分之间的差异具有明显统计学意义(F=2.788,P=0.045),Spearman相关性分析结果显示Tomm34的表达水平与FIGO分期存在一定相关性(r=0.2749,P=0.0073),FIGO分期越高,Tomm34的表达水平越高。Logistic回归分析可得,Tomm34表达水平与CA125水平有显著相关性(r=0.4005,P<0.05),随着CA125水平增高,Tomm34表达水平也越高,即两者之间呈正相关。结论卵巢癌组织中Tomm34表达发生异常,其表达水平与肿瘤类型、分期等有明显相关性。

Objective To study the expression of Tomm34 in ovarian cancer and to explore the relationship between its expression level and cancer stage.Methods The data of 94 patients diagnosed with ovarian cancer in our hospital and treated with surgery from February 2016 to October 2018 were collected for this study.The expression of Tomm34 in tumor was detected by immunohistochemistry.The relationship between clinical and histological features and the Tomm34 expression level was compared.Results After the Tomm34 IHC experiment,11 samples(11.70%)scored 0 points,29 samples(30.85%)scored 1 point,32 samples(34.04%)scored 2 points,and 22 samples(23.40%)scored 3 points.Compared with the Tomm34 level of the two pathological classification samples,the Tomm34 expression score of the first sample(1.49±0.92)was significantly lower than that of the second sample(1.91±0.97),and the difference was statistically significant(P<0.05).Compared with the samples of wild-type p53,ovarian cancer samples with mutated p53 showed significantly higher Tomm34 expression score(P<0.05).The difference between the expression scores of Tomm34 in ovarian cancer samples with different FIGO stages was statistically significant(F=2.788,P=0.045).Spearman correlation analysis results showed that there was a certain correlation between the expression level of Tomm34 and FIGO stage(r=0.2749,P=0.0073),the higher the FIGO stage,the higher the expression level of Tomm34.Logistic regression analysis showed that the expression level of Tomm34 was significantly correlated with the level of CA125(r=0.4005,P<0.05).With the increase of CA125 level,the expression level of Tomm34 was also higher.There is a positive correlation between them.Conclusion The expression of Tomm34 in ovarian cancer is abnormal,and its expression level is significantly correlated with tumor type and stage.