喉癌患者PAC-1、CD62P表达与临床病理特征和复发的关系

Expression of platelet PAC-1,CD62P and its correlation with pathological features and recurrence in laryngeal cancer

目的探讨喉癌患者血小板表面血小板膜糖蛋白Ⅱb/Ⅲa纤维蛋白原受体(PAC-1)、血小板P-选择素(CD62P)阳性表达率以及与患者临床病理特征和复发的关系。方法选取2014年1月~2015年12月间在我院耳鼻喉科手术治疗的116例喉癌患者,随访≥2年,并选取同期在我院体检的健康人群60例为对照组,采用流式细胞仪检测法检测外周血PAC-1和CD62P阳性率,并分析与临床病理特征、复发的关系。结果喉癌患者PAC-1和CD62P阳性表达率分别为(17.82±1.76)%和(22.87±3.13)%,明显高于健康人群(P<0.05);而且在喉癌患者PAC-1表达和CD62P表达呈正相关性(r=0.238,P<0.05)。T3-T4分期或N2-N3分期患者PAC-1和CD62P阳性表达率高于T1-T2分期或N0-N1分期患者(P<0.05)。另外远处转移组PAC-1和CD62P阳性表达率高于未发生转移组(P<0.05);随访期间有24例患者复发,复发率为20.69%。复发喉癌患者PAC-1、CD62P阳性表达率分别为(17.02±0.85)%和(21.84±1.17)%,明显高于未复发的喉癌患者(P<0.05)。经Logistics回归分析,PAC-1和CD62P是喉癌患者复发的独立危险因素(P<0.05)。结论PAC-1和CD62P阳性表达率与喉癌患者T分期、淋巴结转移和远处转移密切相关,同时可作为喉癌局部复发、区域淋巴结转移、远处转移的预测指标。

Objective To investigate the positive expression rate of platelet PAC-1,CD62P and its correlation with pathological features and recurrence in laryngeal cancer.Methods 116 patients with laryngeal cancer who underwent surgery in our hospital from January 2014 to December 2015 were selected as the experimental group,and 60 healthy people who were examined in our hospital during the same period were selected as the control group.The positive rates of platelet PAC-1,CD62P were detected by flow cytometry(FCM).The relationship with clinical pathological features and recurrence were analyzed.Results The positive rates of PAC-1 and CD62P in the experimental group were(17.82±1.76)%and(22.87±3.13)%,which were significantly higher than those in the control group(P<0.05).The positive rates of PAC-1 and CD62P expressions in patients with T3-T4 stage or N2-N3 stage were statistically higher than those in patients with T1-T2 stage or N0-N1 stage(P<0.05).Besides,the positive rates of PAC-1 and CD62P expressions in patients with metastasis were statistically higher than those in patients without metastasis(P<0.05).During the follow-up period,24 patients relapsed,and the recurrence rate was 20.69%.The positive expression rates of PAC-1 and CD62P in patients with recurrence were(17.02±0.85)%和(21.84±1.17)%,which were statistically higher than those in patients without recurrence(P<0.05).Logistics regression analysis showed that PAC-1 and CD62P were the independent risk factors of recurrence(P<0.05).Conclusion The positive rates of PAC-1 and CD62P levels are closely related to the clinical pathological features,recurrence and metastasis of laryngeal cancer.It has important clinical significance for the diagnosis of recurrence and metastasis of laryngeal cancer and its prognosis.