异养硝化-好氧反硝化菌HY3-2的分离及脱氮特性

Isolation and denitrification characteristics of heterotrophic nitrification-aerobic denitrification bacterium HY3-2

从经过高盐驯化的好氧颗粒污泥系统中筛选出一株异养硝化-好氧反硝化菌HY3-2,通过形态学观察及16S rDNA序列分析得出HY3-2为Klebsiella quasipneumoniae subsp.quasipneumoniae.研究了HY3-2对氨氮、硝酸盐和亚硝酸盐的去除特性,结果表明该菌具有良好的异养硝化和好氧反硝化功能,对氨氮、硝酸盐和亚硝酸盐的去除率分别达63.57%、88.11%和98.38%.对菌株脱氮性能研究表明:HY3-2以甘油为碳源,C/N为25,温度为20℃或30℃,转速为150r/min,盐度低于50g/L时,对100mg/L的NH4^+-N去除效果良好,去除率达90.7%;以柠檬酸钠为碳源,C/N为25,温度为30℃,转速为150r/min,盐度低于15g/L时能进行良好的好氧反硝化作用,NO3--N去除率达99%以上.

A heterotrophic nitrification-aerobic denitrification bacterium named HY3-2 was isolated from the high-salt aerobic granular sludge system.The morphological observation and 16S rDNA sequence analysis showed that HY3-2 was Klebsiella quasipneumoniae subsp.quasipneumoniae.The removal characteristics of ammonia nitrogen,nitrate and nitrite by HY3-2 were studied.The results showed that the strain had good heterotrophic nitrification and aerobic denitrification performance,and the removal rates of ammonia nitrogen,nitrate and nitrite were 63.57%,88.11% and 98.38%respectively.The study on the nitrogen removal performance of the strain showed that:HY3-2 used glycerol as carbon source.Under the condition(C/N 25,temperature 20℃ or 30℃,rotation speed 150r/min,initial pH 7~10,salinity less than 50g/L),the effect on NH4+-N removal with the concentration of 100mg/L was good,and the removal rate was 90.7%.When the sodium citrate was used as carbon source(C/N 25,temperature 30℃,rotation speed 150r/min,initial pH 5~9),the aerobic denitrification can be performed when the salinity was less than 15g/L,the NO3^--N removal rate was over 99%.