Ag/石墨烯复合涂层的制备及抗菌性能

Preparation and Antibacterial Properties of Ag/Graphene Composite Coating

首先用改进的Hummers法制备氧化石墨烯(GO);其次用原位还原法将银氨溶液中的Ag纳米粒子通过还原剂葡萄糖和GO复合,获得Ag/GO胶体;最后在真空干燥条件下得到Ag/GO涂层,并用真空阶梯热还原技术制备Ag/还原氧化石墨烯(rGO)涂层。利用X射线衍射、扫描电子显微镜、透射电子显微镜等方法对膜样品结构形貌进行表征,并用润湿角测量仪和抑菌环方法分别检测样品的亲/疏水性和抗菌性能。实验结果表明:石墨烯基膜材料与基底结合较好,Ag纳米粒子在石墨烯片层间呈球形均匀分布,粒径为20~50nm;纯GO和rGO膜表面未见菌落,大肠杆菌与金黄色葡萄球菌均未出现有效抑菌环;复合Ag纳米粒子后,涂层的抑菌效果得到显著提高;与Ag/GO复合膜相比,Ag/rGO复合膜呈更强的抗菌活性,即低温退火有助于提高石墨烯基复合涂层的抗菌性能。

Firstly,graphene oxide(GO)was prepared by modified Hummers method.Secondly,Ag/GO colloids were synthesized from GO and using glucose as reducing agent to reduce silver nanoparticles in silver ammonia solution.Finally,Ag/GO coating was obtained under vacuum drying condition,and Ag/reduced graphene oxide(rGO)coating was prepared by vacuum step-by-step thermal reduction technology.The structure and morphology of the films were characterized by X-ray diffraction,scanning electron microscopy and transmission electron microscopy.The hydrophilic/hydrophobic and antibacterial properties of the samples were tested by wetting angle tester and inhibition zone method.The results show that the graphene-based coatings have a good combination with the substrate,Ag nanoparticles are spherical shape and uniformly distributed between the layers of graphene sheets,and the particle size range is 20-50 nm.No bacterial was observed on the surface of GO and rGO films,and no clear zone of inhibition is found in E.coli and S.aureus.Compared with the Ag/GO composite film,Ag/rGO composite film has stronger antibacterial activity,and low temperature annealing is helpful to improve the antibacterial performance of graphene-based composite coating.