基于苍白球局部场电位分析的光遗传技术对帕金森病大鼠的治疗效果评价

Effect of optogenetics on Parkinson’s disease rats based on globuspallidus local field potential analysis

目的从电生理角度评价光遗传技术对帕金森病大鼠的治疗效果。方法将28只Sprague Dawley大鼠随机分为实验组(n=18)和对照组(n=10),实验组大鼠脑部黑质致密部立体定位注射鱼藤酮溶液制备帕金森病模型,造模成功大鼠注射腺相关病毒并进行光刺激治疗;对照组大鼠黑质致密部立体定位注射生理盐水。通过微电极阵列采集2组大鼠苍白球脑电信号并计算C0复杂度和能量百分比,比较2组大鼠光刺激前后C0复杂度和能量百分比。结果实验组大鼠光刺激前α、β、θ波能量百分比小于对照组,δ波能量百分比大于对照组(P<0.05)。实验组大鼠光刺激中α、β、θ波能量百分比高于光刺激前,δ波能量百分比低于光刺激前(P<0.05)。实验组大鼠光刺激后δ波能量百分比高于光刺激中(P<0.05);其余波段光刺激后与光刺激中比较差异均无统计学意义(P>0.05)。实验组大鼠光刺激前α、β、δ、θ波C0复杂度均小于对照组(P<0.05)。实验组大鼠光刺激中各波段C0复杂度均大于光刺激前(P<0.05);实验组大鼠光刺激中各波段C0复杂度与光刺激后比较差异均无统计学意义(P<0.05)。结论光遗传技术能够改善苍白球脑区神经元放电症状,但光遗传技术单次作用时间较短,光刺激结束后苍白球脑区神经元放电又出现紊乱。

Objective To evaluate the therapeutic effect of optogenetics on Parkinson’ s disease( PD) rats from the perspective of electrophysiology. Methods Twenty eight Sprague Dawley rats were randomly divided into experimental group( n = 18) and control group( n = 10);the rats in the experimental group were injected with rotenone solution in the substantianigra pars compacta( SNc) to eatablish the PD model. The PD rats were injected with adeno-associated virus and were treated by light wave stimulation. The ratgs in the control group were injected with normal saline in the SNc. The local field potential in theglobus pallidus( GP) of the rats were collected by microelectrode array,and the C0 complexity and energy percentage were calculated. The C0 complexity and energy percentage of rats were compared between the two groups before and after light stimulation. Results Before light stimulation,the energy percentages of α,β,θ wave of rats in the experimental group were lower than those in the control group( P < 0. 05),and the energy percentage of δ wave of rats in the experimental group was higher than that in the control group( P < 0. 05). The energy percentages of α,β,θ wave of rats in light stimulation were higher than those before light stimulation and the energy percentage of δ wave was lower than that before light stimulation in the experimental group( P < 0. 05). The energy percentage of δ wave of rats after light stimulation was significantly higher than that in light stimulation in the experimental group( P < 0. 05);there was no significant difference in the energy percentage of other wave in the experimental group( P > 0. 05). The C0 complexity of α,β,δ,θ wave of rats in the experimental group were lower than those in the control group before light stimulation( P < 0. 05). The C0 complexity of all wave in light stimula tion were greater than those before light stimulation in the experimental group( P < 0. 05). There was no significant difference in the C0 complexity of all wave in light stimulation and after light stimulation in the experimental group( P < 0. 05). Conclusion Optogenetics can improve the discharge symptoms of neurons in pallidoglobular brain area,but the single action time of optogenetics is short,and the discharge of neurons in pallidoglobular brain area appears disorder after the end of photostimulation.