柳州市区及周边地区临床分离非结核分枝杆菌的菌种分布研究

Study on species distribution of clinically isolated non-tuberculosis Mycobacterium in Liuzhou City and surrounding area

目的分析柳州市区及周边地区近年非结核分枝杆菌(NTM)临床分离率和菌种分布特征,掌握该地区NTM优势菌群,为NTM感染的临床诊治工作提供参考依据。方法选取2016年3月至2019年4月柳州市区及周边地区分枝杆菌临床分离株1033株,经PNB法初步鉴定,获得NTM菌株184株,再用微阵列基因芯片法行进一步的菌种鉴定。结果184株NTM经微阵列基因芯片法鉴定,检测出9种NTM,其中鸟分枝杆菌占比最大,为50.5%,其次是龟/脓肿分枝杆菌,占27.1%,胞内分枝杆菌占8.2%,戈登分枝杆菌占3.8%,堪萨斯分枝杆菌占3.3%,偶然分枝杆菌、浅黄分枝杆菌、苏尔加/玛尔摩分枝杆菌各占2.2%,土分枝杆菌占0.5%。184例NTM感染患者中,85.9%患者年龄为40岁及以上。结论鸟分枝杆菌和龟/脓肿分枝杆菌为柳州市区及周边地区近年流行的NTM菌种,中老年人为NTM的易感人群,NTM主要引起肺部感染。

Objective To study the clinical separation rate and bacterial species distribution characteristics of non-tuberculosis Mycobacterium(NTM)in Liuzhou City and surrounding area in recent years to master the dominant NTM flora in this area to provide a reference basis for clinical diagnosis and treatment work of NTM disease.Methods A total of 1033 clinically isolated strains of Mycobacterium in Liuzhou City and surrounding areas from March 2016 to April 2019 were selected,after preliminary identification by using the PNB method,184 strains of NTM were obtained,and conducted the species identification by using the microarray gene chip method.Results One hundred and eighty-four strains of NTM were identified by using the microarray gene chip method,and 9 specieses of NTM were identified,in which Mycobacterium avian accounted for the largest proportion(50.5%),followed by Mycobacterium tortoise/abscess,accounting for 27.1%,and Mycobacterium intracellular for 8.2%,Gordon Mycobacterium for 3.8%,Kansas Mycobacterium for 3.3%,Accidental Mycobacterium,Mycobacterium flavum and Suerjia/Maermo Mycobacterium for 2.2%,and Mycobacteria for 0.5%.Among 184 cases of NTM infection,85.9% of the patients were over 40 years old.Conclusion Mycobacterium avian and Mycobacterium tortoise/abscess are the dominant NTM species in Liuzhou City and surrounding area in recent years.The middle-aged and elderly people are susceptible to NTM infection,which mainly causes lung infection.