基于液相色谱-质谱联用技术的代谢组学分析方法研究进展

Advances of Analytical Methods for Liquid Chromatography-Mass Spectrometry-based Metabolomics

代谢组学是研究小分子代谢物的有用工具,能够直接反映生命体终端和表型信息,在精准医学和转化医学中发挥着重要作用。色谱-质谱联用技术具有灵敏度高、选择性好、动态范围宽、信息丰富等优点,已成为代谢组学研究的主要技术平台。代谢组学分析方法的创新与进展是代谢组学在各领域广泛应用的重要前提。该文综述了近5年来基于液相色谱-质谱联用技术的代谢组学分析方法取得的成果,并对目前存在的问题及发展前景给予展望。综述引用文献81篇。

Metabolomics is a powerful tool for small molecule metabolites analysis,and it is playing a more and more important role in the precision medicine and translational medicine.Mass spectrometry(MS)coupled with different chromatographic modes have become the pillar technology platforms for metabolomics due to their high sensitivity,good selectivity,wide dynamic range and rich information.Innovations and developments of these analytical methods are the important prerequisites for wide applications of metabolomics in various fields.Advances of the analytical methods focused on liquid chromatography-mass spectrometry-based metabolomics technique in the past five years are summarized in this review,and the existing problems and future perspectives of this technique are also discussed.81 references are cited.